Table 1.
Chromosome content of subgenomic DNA samples prepared from chromosomes flow-sorted from peaks on flow karyotypes of Ae. umbellulata (MvGB470), Ae. comosa (MvGB1039), Ae. biuncialis (MvGB382) and Ae. geniculata (AE1311/00).
Figure 1.
Differences in the yield of a 173 bp PCR product of the X1N marker amplified from the genomic DNA and subgenomic DNA samples from peaks I–IV on the flow karyotype of Aegilops umbellulata (MvGB470).
Table 2.
COS markers showing polymorphic (≥2 bp) PCR amplicons between wheat and Aegilops species are considered as suitable for the marker-assisted introgression into hexaploid wheat of the U and M genome chromosomes from Ae. umbellulata (UU) and Ae. comosa (MM) and from Ae. biuncialis and Ae. geniculata.
Table 3.
Duplications in the U and M genomes identified in diploid progenitors (Ae. umbellulata and Ae. comosa) and in their tetraploid hybrids Ae. biuncialis and Ae. geniculata by COS markers assigned to Aegilops chromosomes.
Figure 2.
Brachypodium–wheat–Aegilops orthologous relationships from the genomic perspective of Brachypodium distachyon.
The physical positions of the source ESTs of the COS markers are indicated on the Brachypodium chromosomes (Left). Each marker assigned to chromosomes of the wheat D genome or to the chromosomes of Ae. umbellulata (U), Ae. comosa (M), Ae. biuncialis (Ub, Mb) and Ae. geniculata (Ug, Mg) is colour-coded according to the homoeologous groups of Triticum/Aegilops chromosomes. Gaps between two markers assigned to the same Triticum/Aegilops chromosomes are filled in to show synteny (lighter colours). Blocks (designated I–V) indicate Brachypodium genomic regions related to the regions in the U genomes involved in evolutionary genome rearrangements relative to the wheat D genome or to M genomes. When a marker mapped to more than one wheat or Aegilops chromosome, other colour-coded locations are positioned adjacent to the first one. Asterisks indicate the predicted chromosomal location of a locus when the PCR amplicon was specific for the U or M genomes and could be determined unambiguously in at least one Aegilops species (in the diploid progenitor, or in Ae. biuncialis or Ae. geniculata) and when the highest PCR product yield in the other two species was detected in the subgenomic DNA sample containing the same chromosome.
Table 4.
Syntenic genome relationships between the chromosomes of U and M genomes in the diploid progenitors Ae. umbellulata and Ae. comosa and their allotetraploid hybrids Ae. biuncialis and Ae. geniculata, and the chromosomes of rice (R) and Brachypodium (Br).
Table 5.
Conserved genomic regions between the D genome of hexaploid wheat and the chromosomes of the U and M genomes of the diploid species Ae. umbellulata and Ae. comosa and their allotetraploid hybrids, Ae. biuncialis and Ae. geniculata.