Table 1.
Primers and annealing temperatures (temp) for Notch and VEGF pathway molecules.
Table 2.
VEGF and Notch pathway molecules RNA levels in missed abortion and induced abortion.
Figure 1.
Analysis of Dll4/Notch1 and HIF-1a-VEGF signaling molecules using Real-time RT-PCR.
A. VEGF expression was significantly down-regulated in missed abortion compared with induced abortion. B, C, D. VEGFR1, Notch1 and HIF-1a expression were significantly up-regulated in missed abortion compared with induced abortion. E, F. VEGFR2 and Dll4 expressions were higher in missed abortion than in induced abortion, whereas no statistical difference was observed.
Figure 2.
Analysis of Dll4 using immunohistochemistry.
The protein expression of Dll4 was up-regulated in missed abortion (A) than that in induced abortion (B).
Figure 3.
Correlation analysis in missed abortion.
A. the expression of VEGFR1 positively correlated with Notch1 in missed abortion; B. VEGFR2 expression positively correlated with Notch1 in missed abortion; C. Dll4 expression positively correlated with Notch1 in missed abortion; D. HIF-1a positively correlated with Notch1 in missed abortion; E. HIF-1a was closely negatively correlated with VEGF in missed abortion.
Figure 4.
Correlation analysis in induced abortion.
A.VEGF positively correlated withVEGFR1. B.VEGF expression close positively correlated with VEGFR2. C.VEGFR1 expression close positively correlated with VEGFR2. D-,G.Dll4 positively correlated with VEGF,VEGFR1, VEGFR2, Notch1. H,I. HIF-1a positively correlated with VEGFR2 or Dll4.