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Table 1.

Primer sequences used for qRT-PCR analysis of selected genes in hamsters.

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Table 2.

Polyphenol, flavonoid and antioxidant activities of the ethanol extract of T. indica fruit pulp.

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Figure 1.

Chromatogram of T. indica fruit pulp analysed by HPLC.

HPLC analysis was carried out on the hydrolysed sample of T. indica fruit pulp using a reversed-phase column (NovaPak C18, 150 x 3.0 mm, inner diameter 4 µm). Separation of polyphenols was achieved using a linear gradient system comprising of acetonitrile in trifluoroacetic acid (pH2.6) as the mobile phase. Absorbance was measured at 260 nm.

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Table 3.

Food consumption, body weight and liver weight of control and hamsters treated with T. indica fruit pulp extracts, in the presence or absence of cholesterol.

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Table 4.

Analyses of biochemical parameters of control and hamsters treated with T. indica fruit pulp extracts, in the presence or absence of cholesterol.

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Table 5.

Serum antioxidant enzymes, antioxidant activities and lipid peroxidation in control and hamsters treated with T. indica fruit pulp extracts, in the presence or absence of cholesterol.

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Table 6.

Hepatic antioxidant enzymes, antioxidant activities and lipid peroxidation in control and hamsters treated with T. indica fruit pulp extracts, in the presence or absence of cholesterol.

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Figure 2.

The effect of ethanolic extract of T. indica fruit pulp on the expression of selected hepatic genes in hamster (n = 3).

Each bar represents mean ± standard error of mean. Values not sharing a common superscript letter differ significantly at p<0.05. Group I: standard chow plus distilled water (5 ml/kg); Group II: standard chow plus 500 mg/kg T. indica fruit pulp extract; Group III: high-cholesterol diet plus distilled water (5 ml/kg). Group IV: high-cholesterol diet plus 500 mg/kg T. indica fruit pulp extract.

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