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Figure 1.

Structure of (a) amsacrine (m-Amsa) and (b) o-Amsa.

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Figure 1 Expand

Figure 2.

Stacked view of FTIR spectra of ctDNA (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10(d).

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Figure 2 Expand

Figure 3.

Overlaid view of second derivative spectra of ctDNA and duplexes with their complexes of amsacrine at molar ratio of 1/10 (drug/nucleotide).

Panel 1: ctDNA (blue) and complex (red), Panel 2: (AAAAAAAAAAAA)2 (blue) and complex (red) (b), Panel 3: (ATATATATATAT)2 (blue) and complex (red), Panel 4: (TTAATTAATTAA)2 (blue) and complex (red), Panel 5: (AGAGCAACAGAG)2 (blue) and complex (red), Panel 6: (AGAGACCAGAGA)2 (blue) and complex (red).

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Figure 3 Expand

Figure 4.

Close-up view of the FTIR spectra of ctDNA and duplexes with their complexes of amsacrine at 1/10 molar ratio (drug/nucleotide) in the 950-700 cm−1 spectral range.

Panel 1: ctDNA (a) and complex (b), Panel 2: (AAAAAAAAAAAA)2 duplex (a) and complex (b), Panel 3: (ATATATATATAT)2 duplex (a) and complex (b), Panel 4: (TTAATTAATTAA)2 duplex (a) and complex (b), Panel 5: (AGAGCAACAGAG)2 duplex (a) and complex (b), Panel 6: (AGAGACCAGAGA)2 duplex (a) and complex (b).

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Figure 4 Expand

Figure 5.

Stacked view of FTIR spectra of (AAAAAAAAAAAA)2 duplex (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10 (d).

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Figure 5 Expand

Figure 6.

Stacked view of FTIR spectra of (ATATATATATAT)2 duplex (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10 (d).

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Figure 6 Expand

Figure 7.

Stacked view of FTIR spectra of (TTAATTAATTAA)2 duplex (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10 (d).

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Figure 7 Expand

Figure 8.

Stacked view of FTIR spectra of (AGAGCAACAGAG)2 duplex (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10 (d).

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Figure 8 Expand

Figure 9.

Stacked view of FTIR spectra of (AGAGACCAGAGA)2 duplex (a) and its complexes with amsacrine at different molar ratios (r) of 1/50 (b), 1/20 (c) and 1/10 (d).

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Figure 9 Expand