Table 1.
The numbers of CON, CR and HC rats at the age of 3-month and 24-month.
Table 2.
Effect of diet on metabolic parameters and renal function in the aged rats.
Figure 1.
PAS staining of the renal tissues from the CON, CR and HC Fischer 344 rats.
CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet. Magnification, ×400.
Figure 2.
LC3 was decreased in HC kidneys and increased in CR kidneys compared with CON kidneys.
LC3 expression was analysed by Western blotting in the renal tissues of the CON, CR and HC Fischer 344 rats. a: Theexpression of LC3-I and LC3-II proteins was detected by Western blot analysis.b: The ratio of the LC3-II to LC3-I bands was analyzed. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON.c: Immunohistochemistry staining results for LC3 proteins and 8-OHdG in the kidneys of the CON, CR and HC Fischer 344 rats were scanned by a microscope.CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 3.
P62 was decreased in HC kidneys and increased in CR kidneys compared with CON kidneys.
The expression of p62/SQSTM1 and polyubiquitin aggregates was analyzed by Western blotting in kidneys from CON, CR, and HC Fischer 344 rats. a: The expression of p62/SQSTM1 was detected by Western blotting. b: Quantitative analysis of the band density for p62/SQSTM1.c: The expression of polyubiquitin aggregates (poly-UB) was analyzed by Western blotting. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON. CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 4.
PINK1 was decreased in HC kidneys and increased in CR kidneys compared with CON kidneys.
The expression of Parkin and PINK1 was analyzed by Western blotting in the kidneys of CON, CR, and HC Fischer 344 rats. a: The expression of Parkin and PINK1 was detected by Western blotting. b:Quantitative analysis of the band density for Parkin. c:Quantitative analysis of the band density for PINK1. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON. CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 5.
Bnip3, Ambra1 decreased in HC kidneys and increased in CR kidneys compared with CON kidneys.
The expression of Bnip3 and Ambra1was analyzed by Western blotting in the kidneys of CON, CR, and HC Fischer 344 rats. a: The expression of Bnip3 and Ambra1 was detected by Western blotting. b:Quantitative analysis of the band density for Bnip3. c:Quantitative analysis of the band density for Ambra1. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON.CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 6.
The damage of mitochondrial structures was increased in HC kidneys and decreased in CR kidneys.
Analysis of mitochondrial structures and autolysosomes by transmission electron microscopy (TM) in the renal tissues of CON, CR, and HC Fischer 344 rats. White arrows indicate damagedmitochondria; black arrows indicate autolysosomes.CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 7.
The level of 8-OHdG was increased in HC kidneys and decreased in CR kidneys.
Immunohistochemistry staining results for LC3 proteins and 8-OHdG in the kidneys of the CON, CR and HC Fischer 344 rats were scanned by a microscope. CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.
Figure 8.
The levels of p16 and SA-β-gal increased in HC kidneys and decreased in CR kidneys.
The expression of the senescence biomarkers p16 and senescence-associated-galactosidase wasanalyzed in the kidneys of CON, CR, and HC Fischer 344 rats. a: Western blot results for p16 protein. b: Quantitative analysis of the band density for p16. The protein expression data are presented as the mean ± SD (n = 6). *p<0.05 vs. CON. c:Senescence-associated-galactosidase staining (magnification ×400). Blue precipitation in the cytoplasm was observed in the senescent cells.CON, control animals; CR, calorie-restricted diet; HC, high-calorie diet.