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Figure 1.

Melanoma in association with a nevus in overview (A) and closeup (B).

Immunohistochemically stained in (C) with the BRAFV600E-mutation specific antibody VE1. Benign(D) and malignant(F) parts of the tumor in closeup. E&G show sequencing result of the corresponding cuts.

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Figure 1 Expand

Figure 2.

Comparison of mutation status between paired tumor groups.

BRAFV600E-mutation status was evaluated by VE1-Immunohistochemistry and Sanger sequencing, NRASQ61 by Sanger sequencing alone. p-values denote two-tailed significance as measured by McNemar test.

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Figure 2 Expand

Figure 3.

Representative tumor parts of nevi groups.

Melanoma associated nevi are more commonly strictly dermal and show less of the postulated features of so called "dysplastic" nevi.

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Figure 3 Expand