Table 1.
Patient characteristics.
Table 2.
Primer sequences.
Figure 1.
Graphical representation of normalized expression values.
(A–H) Box plots representing the distribution of normalized expression values of normal (n = 30), tumor (n = 60) and CC cell lines (n = 8). A box in a given box plot represents the interquartile range (25th percentile to 75th percentile), the middle line denotes median and the extreme ends of the whiskers marks the minimum and maximum values. P-values indicated over each box represent the asymptotic significance (2-tailed) of Mann-Whitney test comparing normal to tumor and normal to cell lines independently.
Figure 2.
Expression of MCM genes in different tumor stages.
Normalized expression level of (A) MCM2, (B) MCM4, (C) MCM5, (D) MCM6, (E) MCM10 and (F) RECQL4 in different tumor stages. I, II, III denote Stage I (n = 06), Stage II (N = 22) and Stage III (n = 32) respectively. Each axis represents mean ± SD.
Figure 3.
Frequency of over expression of MCM genes with advancing stage of disease.
Percentage of cases showing over expression of (A) MCM2, (B) MCM4, (C) MCM5, (D) MCM6, (E) MCM10 and (F) RECQL4 in each tumor stage. I, II, III denote Stage I (n = 06), Stage II (N = 22) and Stage III (n = 32) respectively.
Figure 4.
Distribution of expression of MCMs in different types of lesion of cervical cancer.
Differential expression of (A) MCM4; (B) MCM5; (C) MCM6 in different types of lesions viz. infiltrative (n = 08), ulceroinfiltrative (n = 11), proliferative (n = 21), ulceroproliferative (n = 09), proliferative (n = 11) as compared to normal; (D). MCM10 show significant up regulation compared to normal in all different types of lesions. A box in a given box plot represents the interquartile range (25th percentile to 75th percentile), the middle line denotes median and the extreme ends of the whiskers marks the minimum and maximum values. P-values indicated over each box represent the asymptotic significance (2-tailed) of Mann-Whitney test comparing normal to a particular group.
Figure 5.
(A) Electrophoregram of semi-quantitative RT-PCR showing MCM4 expression in cell lines, primary tumors (CC1–CC5) and normals (N1-5). (B) Immunodetection of MCM4: Expression of MCM4 protein in cell lines; normals (N1-5) and tumors (CC1-5).
Figure 6.
Immunohistochemical analysis of MCM4 expression in (A) Normal cervix; (B) Cancerous cervix; Ki67 expression in (C) Normal cervix; (D) Cancerous cervix (60X).