Table 1.
EGFR mutational analysis of lung adenocarcinoma, malignant melanoma and colorectal carcinoma – Sanger/q-PCR sequencing versus NGS.
Table 2.
BRAF and KRAS mutational analysis of lung adenocarcinoma, malignant melanoma and colorectal carcinoma by NGS LA: lung adenocarcinoma; MM: malignant melanoma; CRC: colorectal cancer.
Figure 1.
Distribution of variants detected using the Ion AmpliSeq Cancer Panel.
A heat map was generated illustrating the variants occurring in all 46 genes by both IonTorrent software versions in each of the clinical samples. COSMIC tracked variants are also described.
Table 3.
NGS gene mutation detection using the Ion AmpliSeq Cancer Panel in lung adenocarcinoma.
Table 4.
NGS gene mutation detection using the Ion AmpliSeq Cancer Panel in colorectal carcinoma.
Figure 2.
Variant frequency between thresholds with Cosmic IDs associated.
The graph illustrates the importance of filtering mutations by applying different detection thresholds in the bioinformatics analysis. ID.1, 2, 3, 6 and 9 were selected as they contained important standard-of-care SNVs.