Figure 1.
Locations from where the plants of Lepidium latifolium were collected from Ladakh region of Jammu and Kashmir State, India; Kargil (▪), Leh (•), Nyoma (▴). The figure is for illustrative purposes only.
Table 1.
Relative proportion of methanolic fraction in different position of leaves and roots of Lepidium latifolium expressed as percentage of total amount of crude extract used for the fractionation (n = 3).
Figure 2.
Metabolic content of eight reference glucosinolates in leaves of Lepidium latifolium at three different locations of Kargil, Leh and Nyoma.
* represents statistically significant values at p≤0.05.
Figure 3.
Representation of the major glucosinolates in native plant species of Lepidium latifolium in Ladakh.
Values represent the average of all the three locations.
Table 2.
Percentage (%) fatty acid composition of Lepidium latifolium from different locations of Ladakh.
Table 3.
Elemental analysis in leaves and roots of Lepidium latifolium from three different locations of Ladakh.
Figure 4.
Total glucose content as observed in Kargil (□), Leh (∥∥) and Nyoma (▪) by the action of glucose oxidase.
Means with the different letter represents statistically significant values at p≤0.05 using ANOVA.
Figure 5.
Total crude protein as observed in Kargil (□), Leh (∥∥) and Nyoma (▪) using Bradford reagent.
Means with the different letter represents statistically significant values at p≤0.05 using ANOVA.
Table 4.
Antioxidant capacity in Lepidium latifolium suggested by total phenols, total flavanoids, reducing and chelation power in different leaf positions and roots at three different locations of Ladakh.
Figure 6.
Antioxidant activity of methanolic plant extracts of Lepidium latifolium at Kargil (□), Leh (∥∥) and Nyoma (▪).
Panel ‘A’ represents superoxide, panel ‘B’ represents hydroxyl and panel ‘C’ represents DPPH radicals. Antioxidant activity was represented as % inhibition of respective free radicals * represents statistically significant values at p≤0.05.
Figure 7.
DNA protective activity of methanolic plant extracts of Lepidium latifolium.
Panel ‘A’ represents 1.2% agarose gel stained with ethidium bromide visualised under UV whereas, panel ‘B’ represents Integrated Density Values (IDV) of bands using densitometry analysis. P: plasmid; FR: Fenton's reaction; K: Kargil; L: Leh; N: Nyoma.
Table 5.
Position wise analysis of total protein, phenols, flavanoids and antioxidant capacity of leaves and roots in Lepidium latifolium.