Table 1.
To compare the clinicopathologic characteristics of ALK-positive group respectively with common driver mutation group and pan-negative group.
Figure 1.
Morphology of ALK-rearrangement lung adnenocarcinoma.
(A) papillary and micropapillary patterns; (B) mucinous cribriform pattern consisting of abundant extracellular mucus and cribriform structures; (C) solid pattern with signet ring cells; (D) mucinous cribriform pattern often floating within mucus-filled alveolar spaces; (E) mucous cells in form of goblet cells; (F) solid pattern with hepatoid tumor cells having abundant eosinophilic cytoplasm, round nuclei, and prominent nucleoli; the tumor cell nuclei are relatively monomorphic.
Table 2.
Comparison of RT-PCR, IHC and FISH for detection of EML4-ALK positive lung cancers.
Figure 2.
Two patterns of ALK gene alteration detected by FISH using Vysis LSI ALK Dual Color breakpoint probe.
(A) Two distinct red and green signals. (B) Isolated red signal.
Figure 3.
Immunohistochemical staining with D5F3 and ALK1 detects ALK-rearrangement tumors.
Case 1: (A) score 3+ showing intense granular cytoplasmic staining with D5F3 antibody; (B) score 1+ showing faint cytoplasmic staining with ALK1; Case 2: (C) score 3+ showing intense granular cytoplasmic staining with D5F3; (D) score 2+ showing moderate, granular cytoplasmic staining with ALK1; Case 3: (E) score 3+ showing intense granular cytoplasmic staining with D5F3; (F) score 1+ showing faint, barely discernible cytoplasmic staining with ALK1.
Table 3.
Comparison between RT-PCR and IHC.
Table 4.
Comparison between FISH and IHC.