Figure 1.
Tomographic slice and histological images of normal mouse liver.
(A, D) Phase contrast image of mouse liver tissue obtained without OsO4 staining. The microvascular structure was ambiguous. (B, E) Absorption contrast image of mouse liver tissue with OsO4 staining. The sinusoids appeared as smaller pillars (arrows) and the veins seemed larger circles (arrowheads). (C, F) Histological image of normal mouse liver stained with hematoxylin and eosin (original magnification, ×20). (D–F) Magnification of the hepatic sinusoid region indicated by rectangles in Figure 1A, 1B, and 1C, respectively. Sinusoidal structure was similar between the histological images and absorption contrast X-ray images. Arrowheads indicate veins and arrows indicate sinusoids. Scale bar, 100 µm.
Figure 2.
Tomographic slice and histological images of normal and acute CCl4-injured liver.
Representative histological images of liver tissue section, stained with hematoxylin and eosin, (A) a normal mouse and (B) a mouse treated with CCl4 for 24 h. (original magnification, ×20). Representative coronal tomographic slice images of OsO4-stained (C) normal and (D) CCl4-treated mouse liver. Disrupted sinusoidal structures in necrotic areas were distinguished from non-necrotic regions from acute CCl4-injured mice. Arrowheads indicate veins and arrows indicate sinusoids. Scale bar, 100 µm.
Figure 3.
Tomographic slice and histological images of pericentral sinusoids and central vein.
Pericentral sinusoids and central vein of livers from (A–C) normal and (D–F) CCl4-treated mice. Representative histological images of liver tissue, stained with hematoxylin and eosin, from (A) normal and (D) CCl4-treated mice (original magnification, ×20). Axial tomographic slice images of the pericentral area of OsO4-stained liver tissue segmented into the (B, E) pericentral sinusoids and (C, F) central vein, from normal and CCl4-treated mice. Solid red lines indicate segmented areas of the pericentral sinusoids (B, E) and of the central vein (C, F). Pericentral sinusoids were disrupted, but the central vein was unaffected by CCl4 damage in the CCl4-injured liver. Scale bar, 100 µm.
Figure 4.
Tomographic slice and histological images of periportal sinusoids and portal vein.
Periportal sinusoids and portal vein of livers (A–C) normal and (D–F) CCl4-treated mice. Representative histological images of liver tissue, stained with hematoxylin and eosin, from (A) normal and (D) CCl4-treated mice (original magnification, ×20). Coronal tomographic slice images of the pericentral area of OsO4-stained liver segmented into the (B, E) periportal sinusoids and (C, F) portal vein, from normal and CCl4-treated mice. Solid red lines indicate segmented areas of the periportal sinusoids (B, E) and of the portal vein (C, F). No significant difference in periportal sinusoids and the portal vein was observed between normal and CCl4-injured livers. Scale bar, 100 µm.
Figure 5.
3D volume rendering images of hepatic sinusoids and veins.
Volume rendered images of hepatic sinusoids and veins of (A, C) normal and (B, D) CCl4-treated mice. Three-dimensional images shown according to metabolic zonation of the liver lobule: (A, B) periportal area and (C, D) pericentral area. The portion of the white box was enlarged to show the magnified view of 3D volume-rendered images of periportal and pericentral sinusoids in normal and CCl4-treated mice. The size of the 3D image is 435 × 435 × 145 µm3 before magnification and 36 × 36 × 36 µm3 after magnification. Disrupted 3D structures of pericentral sinusoids were distinguished from undisrupted 3D periportal sinusoids in CCl4-injured liver. Scale bars, 100 µm. Enlarged images scale bars, 10 µm.
Figure 6.
Quantitative analysis of hepatic sinusoids in normal and CCl4-treated liver.
(A) Sinusoidal diameters in pericentral and periportal regions were measured in normal and CCl4-treated mice. (B) The sinusoidal volume (SV)/parenchymal volume (PV) ratio and (C) sinusoidal volume (SV)/sinusoidal surface area (SA) ratio were measured in normal and CCl4-treated mice. Data are represented as mean ± SD. *P< 0.05; ***P< 0.001.