Figure 1.
Schematic principles of A) SDTF synthesis on inner surface PCR tube by ion beam deposition method, B) PCR tubes holder and samples configuration for silicon dioxide IBD.
Figure 2.
SDTF chemical structure and plastic (polypropylene) sample with functional covering scheme.
A) The result of ion etching of thin films layers of Ag(indicator)/SiO2(silicon dioxide)/C(polypropylene)-substrate by Auger electron spectroscopy thin films ion profiling PCR tube inner surface sample. The spectrum indicates the chemical elements surface concentration by results of which is observed the absence of silicon dioxide on the PCR tube inner surface. B) Ag(indicator)/SiO2(silicon dioxide)/C(polypropylene)-substrate surface concentration on inner surface of PCR tube after AES Ion profiling of thin films. The spectrum indicates an increase of the silicon and oxygen peaks. C) Auger electron spectroscopy depth profile of SDTF on graphite substrate. D) The dashes line show the research area of PCR tube inner surface.
Figure 3.
Phase analysis, element distribution mapping of plastic (polypropylene) sample with functional covering and real-time polymerase chain reaction.
A) Raman spectrum of two samples of inner surface PCR tubes with amorphous SDTF prepared by the IBD method under the same conditions. B) Auger electron spectroscopy element distribution mapping. Scale bar is 200 µm. The spectrum color scheme shows distribution of: carbon (C) – upper left scan sector, oxygen (O) – upper right scan sector, silicon (Si) – lower left scan sector and secondary electron image (lower right scan sector). C) Amplification curves obtained using 1– approach, which involves the introduction of a single cell (oocyte) in a tube without silicon dioxide nanocovering; 2– method implied the introduction of a single cell (oocyte) in a tube with silicon dioxide nanocovering, followed by a cell lysis and washing of tubes from impurities. It gave uniform positive results in the course of PCR, which confirms the efficient extraction of DNA from single cells.