Figure 1.
Appearance of specimens and SAXS sampling locations.
A) Left and C) right PK specimens from Patient 1. Red rectangle in C) shows discontinuity of the wound edge in the right eye. B–E) SAXS scans performed on the PK and control specimens.
Figure 2.
SAXS data across the graft-host interface of the PK specimens from Patient 1, showing measurements of collagen fibril spacing (circles), spatial order index (squares) and diameter (triangles) along the scans indicated in Fig.
1. Corresponding data is also shown for control specimens. Arrows indicate scan positions lying on the PK graft margin and their equivalent positions on the controls. Note local increase in fibril spacing and diameter, and accompanying decrease in spatial order, in the graft margin for the PK specimens.
Figure 3.
Polar vector map of preferential collagen fibril alignment across the left PK specimen of Patient 1, determined using WAXS.
The larger plots (indicative of greater fibril alignment) have been scaled down for montage display by factors indicated in the colour key. Broken circle: limbus. Arrow: tangential fibril alignment along the graft margin. Arrowhead: abnormal inward extension of tangential limbal fibrils into the peripheral cornea, corresponding to region of prominent corneal opacity. Solid line: superior tag. Inset: location of noted features on actual specimen.
Figure 4.
Distribution maps of A,D) total and B,E) preferentially aligned collagen across the PK specimens from Patient 1, determined using WAXS (arbitrary units).
Arrows: local elevation in collagen mass at the graft margin. Arrowheads: abnormal elevation of collagen mass in the peripheral cornea, corresponding to the prominent regions of corneal opacity. Solid circles: limbus. Solid lines: superior tag. Rectangles: Elevated collagen mass delineates the separated wound edges of the right PK specimen, with a reduction in the intervening tissue. C,F) Location of noted features on actual specimens.
Figure 5.
Proportion of total collagen oriented within 45° sectors of the horizontal (transverse to the wound edge) and vertical (parallel to the wound edge) directions as a function of distance from the corneal centre in A) Patient 1, left PK specimen (sampling interval: 0.25 mm), B) normal control cornea (sampling interval 0.4 mm) and C) Patient 2, right PK specimen (sampling interval: 0.25 mm).
Note marked increase/decrease in collagen oriented parallel/transverse to the wound edges in A) and C).
Figure 6.
Polar vector map of preferential collagen fibril alignment across the right PK specimen of Patient 1, determined using WAXS.
The larger plots (indicative of greater fibril alignment) have been scaled down for montage display by factors indicated in the colour key. Broken circle: limbus. Arrow: tangential fibril alignment along the graft margin. Arrowhead: abnormal inward extension of tangential limbal fibrils into the peripheral cornea, corresponding to regions of prominent corneal opacity. Rectangle: fibril alignment delineates separated wound edges and is disturbed in intervening tissue. Solid line: superior tag. Inset: location of noted features on actual specimen.