Table 1.
Distribution of subjects included in the study and the corresponding CLL and CLL-like MBL clones, according to diagnosis.
Table 2.
Peripheral blood (PB) B-cell counts and BCR features of clonal MBLlo, MBLhi andCLL B cells.
Table 3.
Cytogenetic and molecular features of MBLlo, MBLhi and CLL B-cell clones.
Table 4.
Molecular characteristicsof the BCR of CLL-like MBLlo, MBLhi and CLL B-cell clones.
Figure 1.
Frequency of CLL-associated cytogenetic alterations (A) and the cytogenetic profile (B) for those IGHV genes most commonly detected in “low-count MBL”(MBLlo), “high-count MBL” (MBLhi) and CLL B-cell clones, as assessed by interphase fluorescence in situ hybridization (iFISH).
The three diagnostic categories studied are depicted by different colors (green, MBLlo; red, MBLhi; blue, CLL B-cell clones) and the absence vs presence of one vs≥2 chromosomal alterationsperclone, is indicated by empty circles, light colored and dark colored circles, respectively. For each IGHV subgroup, the clones are represented in the Y-axis according to the absolute number of clonal B cells per µL of PB (A) and the percentage of cells genetically altered, by iFISH (B). Different FISH patterns are defined by the following symbols in panel B:, del(13q14.3);, biallelic del(13q14.3);, del(13q14);, trisomy 12; Δ, del(11q);▿,del(17p) and; □, t(14q32); dotted contour lines in panel A highlight those clones phenotypically classified as SLL(small lymphocytic lymphoma); dotted blue lines in panel B indicate cells from the same B-cell clone showing different cytogenetic abnormalities; U = unmutated clones; a = clones with NOTCH1 mutation.
Figure 2.
Principal component analysis (3-dimensionalX-Y-Z axis view of PC1 vs PC2 vs PC3, respectively) for comparison of “low-count MBL” (MBLlo), “high-count MBL” (MBLhi) and CLL B-cell clones according to the absolute number of clonal B cells/µL and the pattern of cytogenetic alterations (including the percentage of altered cells), using the InfinicytTMsoftware.
Overall, MBLlo, MBLhi and CLL cases are clustered into groups distinguished by different colors in A: magenta, gray, and black circles (A). The distribution of MBLlo, MBLhi, CLL-stage A and CLL-stage B/C clones are coloured differently in B: MBLlo, green; MBLhi, red, CLL stage A and B/C light blue and dark blue, respectively (B). The most informative parameters contributing to the best discrimination between 1×1 comparisons of the three groups are displayed in a decreasing order of percentage contribution to each of the principal component (C); Distribution of MBLlo, MBLhi and CLL clones among the three major groups defined in panel A by principal component analysis (D); CLL, chronic lymphocytic leukemia; MBL, monoclonal B lymphocytosis; PC: principal component.