Table 1.
Progression of NM-induced skin injury in terms of clinical lesion development in SKH-1 hairless and C57BL/6 mice.
Table 2.
NM-induced skin edema and erythema in SKH-1 hairless and C57BL/6 mice.
Table 3.
NM-induced skin wounding and pigmentation changes, and dry and scaly skin (xerosis) in SKH-1 hairless and C57BL/6 mice.
Figure 1.
Effect of time-dependent NM exposure on the cutaneous toxicity and clinical sequelae of injury progression in SKH-1 hairless (A) and C57BL/6 (B) mice.
Dorsal skin of mice was exposed topically to either 200 µL of acetone or NM (3.2 mg) in 200 µL acetone, and animals were observed for NM-induced clinical effects/lesions on exposed skin tissue. Pictures were taken and the visible injury lesions were compared between SKH-1 hairless (A) and C57BL/6 (B) mice. VC, vehicle control.
Figure 2.
Quantitative evaluation of the cutaneous lesions and clinical sequelae of injury progression following time-dependent NM exposure in SKH-1 hairless and C57BL/6 mice.
Dorsal skin of mice was exposed topically to either 200 µL of acetone or NM (3.2 mg) in 200 µL acetone. After NM exposure, as a function of time, an overall injury progression (A), clinical lesions namely edema and erythema (B), appearance of microblisters (C), wounding and pigmentation changes (D), and scaly and dry (xerosis) skin (E) were scored. These scores were compared in both SKH-1 hairless and C57BL/6 mice as detailed under Materials and Methods, and recorded in Tables 1, 2, and 3. Data presented are mean ± SEM of 5 animals in each group; VC, vehicle control.
Figure 3.
Effect of time-dependent NM exposure on the skin bi-fold thickness in SKH-1 hairless and C57BL/6 mice.
Dorsal skin of mice was exposed topically to either 200 µL of acetone or NM (3.2 mg) in 200 µL acetone. Skin bi-fold thickness was measured as a function of time following NM exposure using a digital caliper as detailed under Materials and Methods. Data presented are mean ± SEM of 2–5 animals in each group. *, p<0.05 compared to respective vehicle control; VC, vehicle control.