Figure 1.
MCA system for size-based isolation of CTCs.
(a) Schematic diagram of the structure of the MCA system. (b) Scanning electron microscope image of a cultured tumor cell line trapped on the MCA system. (c–f) Cells isolated from SCLC patient blood stained with Hoechst 33342 (c) and fluorescent-labeled antibodies that target cytokeratin (d) and CD45 (e). Merging of the images (f) allowed for identification of CTCs and hematologic cells. Scale bar = 60 µm.
Table 1.
CTC recovery efficiency and average cell diameter.
Figure 2.
CTC count using the MCA system.
CTC count/7.5 mL blood is shown for 6 healthy donors, 22 NSCLC patients and 20 SCLC patients.
Table 2.
Patient characteristics.
Table 3.
Comparison of CTC enumeration by the CellSearch system and the MCA system.
Figure 3.
Gallery of cells captured on the MCA from blood of advanced lung cancer patients.
Cells were stained with Hoechst 33342, FITC-labeled anti-cytokeratin antibody, and PE-labeled anti-CD45 antibody.
Figure 4.
Gallery of CTCs captured on a transparent MCA from SCLC patient blood.
May-Grünwald–Giemsa-stained cells showed a high nucleus–cytoplasm ratio and nuclear molding (×40). Black arrow indicates 9-µm microcavity. Scale bar = 60 µm.