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Figure 1.

Distribution of A) Ages in the study cohort, B) DNA methylation levels for autosomal markers in males and females, C) DNA methylation level for autosomal markers in the youngest (age <18, N = 51) and oldest (age>71, N = 52) individuals of the study, and D) DNA methylation levels for X chromosomal markers in males and females.

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Table 1.

Number of modes in the distribution of DNA methylation for each site.

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Table 2.

Principal components for the DNA methylation levels among autosomal markers and corresponding correlation with age and variance.

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Figure 2.

Increase in DNA methylation level with age of one CpG site (cg16867657) in the promoter of the ELOVL2 gene and corresponding regression line.

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Figure 3.

Location of CpG site depending on correlation between DNA methylation level and chronological age.

Observations are ordered by the correlation coefficients and combined into 100 bins. The illustrations show the fraction of markers within each bin with a location in relation to, A) CGIs, island shores and islands shelves, B) Known promoter and enhancer regions, and C) Gene and transcription starting site.

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Figure 4.

Summary statistics for the CGIs depending on the correlation between DNA methylation level and chronological age.

Observations are ordered by the correlation coefficients and combined into 100 bins. The features of the CGIs within each bin is summarized as, A) Mean length of the CGIs, B) Mean percentage of CpGs in the islands, and C) Mean of observed to expected ration of CpGs in the islands.

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Figure 5.

Distribution of DNA methylation sites mapped to regions with different chromatin states as defined by Ernst et al

[21]. DNA methylation marks are ordered by the correlation coefficient with age and combined into 100 bins.

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Figure 5 Expand