Figure 1.
(a) The Bioptigen spectral-domain ophthalmic imaging system (SDOIS) with animal imaging mount - rodent alignment stage (AIM-RAS) components; (b) aerial view of mouse within cassette - white arrow points to right eye where all scans were obtained.
Figure 2.
(a) C57BL/6 mouse en-face scan showing the central optic nerve head;
(b) showing the 1 mm diameter (arrows) within and around central retinal subfield (CSF); (c) the retinal quadrants surrounding CSF with overall mean (±SD) total retinal thickness; (d) a B-scan image from equatorial slice of en-face scan showing the different sublayers that were measured using the manual calipers. [ORL, outer retinal layer; IRL, inner retinal layer; RPE, retinal pigment epithelium; ELM/IS/OS, external limiting membrane/inner segment of photoreceptors/outer segment of photoreceptors; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL/GC, inner plexiform layer/ganglion cell; RNFL, retinal nerve fiber layer].
Table 1.
Mean Total Retinal Thickness and Sublayer Thickness±Standard Deviation (µm).
Table 2.
Mean Global Total Retinal Thickness (µm) and in Different Quadrants of Retina in Each Animal.
Table 3.
Mean Retinal Thickness (µm) in Different Sublayers In Each Animal.
Figure 3.
Bland-Altman plot showing representative inter-observer difference for total retinal thickness (TRT) measurements in the nasal quadrant of all animals (n = 30).
Each black dot in the figure corresponds to a specific TRT measurement point within the B-scan – twelve points were measured in each quadrant. Since the graph depicts TRT values in the nasal quadrant for all 30 animals, in total, 360 TRT measurements are represented in this graph.
Figure 4.
Bland-Altman plot showing inter-observer difference for mean retinal sublayer thickness measurements in 10 randomly selected animals.
Each dot in the figure corresponds to the mean thickness from 10 animals and the color code represents 9 different sublayers: red, retinal pigment epithelium; blue, external limiting membrane/inner segment of photoreceptors/outer segment of photoreceptors; pink, outer nuclear layer; purple, outer plexiform layer; yellow, inner nuclear layer; brown, inner plexiform layer/ganglion cell; green, retinal nerve fiber layer; orange, outer retinal layer; black, inner retinal layer.
Table 4.
Interobserver Correlation In Measurement of SD-OCT Mean Retinal Thickness (µm).