Figure 1.
Illustration of the prominent peaks of polystyrene beads and bladder cancer cells (MGH-U1).
[a] Standard Raman spectrum of the 1001.4 cm−1 peak of polystyrene [b] Wavelength Modulated (WM) Raman spectrum of the 1001.4 cm−1 peak of polystyrene [c] Standard Raman spectrum of the 1453 cm−1 peak of MGH-U1 [d] WM Raman spectrum of the 1453 cm−1 peak of MGH-U1.
Figure 2.
Characteristics of SNR variation with modulation amplitude for polystyrene and MGH-U1.
Table 1.
Illustration of the dependence of SNR on the time constant and the sampling rate.
Figure 3.
Illustration of the typical SNR characteristics.
SNR characteristics, while varying the sampling rate, with progressing total acquisition time with a time constant of 5 s.
Figure 4.
Cluster plot for MGH-U1 and SV-HUC 1.
Plot of principal component 1 and principal component 2 of WM Raman spectra of 50 MGH-U1 cells and 50 control SV-HUC 1 cells for a time constant of 5 s, [a] clusters overlapping at a total acquisition time of 10 s, [b] segregated clusters at a total acquisition time of 15 s, [c] segregated clusters at a total acquisition time of 60 s.
Figure 5.
PCA of WM Raman spectra of 50 MGH-U1 cells and control SV-HUC 1.
The time constant is 2 s and total acquisition time is 6 s.