Figure 1.
Length distribution of Chrysopa pallens unigenes.
All unigene sizes were calculated. The X-axis shows the length (nt) of unigenes. The Y-axis shows the number of unigenes.
Table 1.
Summary of Chrysopa pallens transcriptome.
Figure 2.
Distribution of Chrysopa pallens unigenes annotated by GO.
The X-axis shows three categories and their subcategories. The Y-axis shows the percentage of sequences. The analysis level was 3.
Figure 3.
Species distribution of the BLASTX results.
Unigenes were searched against the NR protein database using BLASTX with a cutoff e-value <10−5 and the proportions of each species (represented by different colors) graphed. Species with proportions of more than 1% are shown.
Figure 4.
Alignment of amino acid sequences of the candidate OBPs and CSPs.
(A): Alignment of amino acid sequences of the candidate OBPs. (B): Alignment of amino acid sequences of the candidate CSPs. Predicted signal peptides are boxed, and the conserved cysteines are highlighted in blue. Sequences which contained a full length open reading frame were labeled by red line.
Figure 5.
Phylogenetic tree of candidate odorant-binding proteins (OBPs) from Chrysopa pallens and five other species.
Tcas: Tribolium castaneum, Dmel: Drosophila melanogaster, Bmor: Bombyx mori, Apis: Acyrthosiphon pisum, Amel: Apis mellifera. The C. pallens unigenes were labeled with ‘CL’ (clusters) and ‘U’ (unigenes). CL-X: CL-contigX.
Figure 6.
Phylogenetic tree of candidate chemosensory proteins (CSPs) from Chrysopa pallens and five other species.
Tcas: Tribolium castaneum, Dmel: Drosophila melanogaster, Bmor: Bombyx mori, Apis: Acyrthosiphon pisum, Amel: Apis mellifera. The C. pallens unigenes were labeled with ‘CL’ (clusters) and ‘U’ (unigenes). CL-X: CL-contigX. Arrow means 8 CSPs representing five of the six species clustered together with a bootstrap percentage >50.
Figure 7.
Phylogenetic tree of candidate cytochrome P450s from Chrysopa pallens and Bombyx mori.
The C. pallens unigenes were labeled with ‘CL’ (clusters) and ‘U’ (unigenes). Bmor: B. mori. CL-X: CL-contigX.
Table 2.
Tag analysis statistics of Chrysopa pallens transcriptome.
Figure 8.
Differentially-expressed unigenes between different tissues of Chrysopa pallens.
(A) Numbers of up- and down-regulated unigenes in each pair-wise comparison of tissues. (B) Numbers of up- and down-regulated unigenes compared between female and male tissues. FA: female antennae, FH: female heads without antennae, FL: female legs, FW: female wings, FB: female thoraxes and abdomens, MA: male antennae, MH: male heads without antennae, ML: male legs, MW: male wings, MB: male thoraxes and abdomen.
Table 3.
Differentially-expressed unigenes between different tissues of Chrysopa pallens.
Figure 9.
Verification of 14 odorant-binding protein (OBP) genes in tissues of female and male Chrysopa pallens.
(A) Gene expression analysis of 14 OBP genes for which DGE data were available. Blue, DGE data; red, qRT-PCR results. (B) Gene expression analysis of four OR genes. The relative expression levels were normalized by GTP-binding protein (U15543) and ribosomal protein (U29931) Error bars represent standard error.