Figure 1.
The length distribution of the assembled transcripts.
Table 1.
Overview of the sequencing and assembly.
Figure 2.
Gene ontology classification of assembled transcripts.
Figure 3.
COG function classification of assembled transcripts.
Figure 4.
Volcano plot of gene expression difference between Pb1000 and CK samples.
Table 2.
Enriched pathways of the up-regulated DEGs.
Table 3.
Enriched pathways of the down-regulated DEGs.
Table 4.
Validation of the RNA-Seq expression profiles of selected DEGs by qRT-PCR.
Figure 5.
qRT-PCR analysis of six selected DEGs with different concentrations and temporal treatments of Pb(NO3)2.
A-F represent different concentrations of Pb(NO3)2 after 72 h treatment and G-L represent different temporal duration of a fixed concentration of Pb(NO3)2 at 1000 mg L−1.
Figure 6.
Expression pattern of genes involved in glutathione metabolism.
The red and green color in each column indicated up- and down-regulation enzymes. The heatmaps generated were colored to the corresponding DEGs from the KEGG database (map 00480).
Table 5.
The identified genes involved in glutathione metabolism of the de novo transcriptome.