Table 1.
Bacterial strains used in this study.
Figure 1.
Examples of differences between the WGMs created in the lab and their in-silico counterparts.
The figure shows details of the comparisons of the maps of LA-MRSA strain S0385 and MRSA strains N315 and NCTC8325. The WGMs are represented as linear maps displaying the fragments in randomly chosen colours, with matching fragments sharing the same colour.
Figure 2.
Detail of the whole genome maps of an outbreak of CA-MRSA (USA300) showing an additional DNA segment in 3 isolates.
All isolates had spa-type t024 and MLVA-type MT308. The gel image on the right hand side shows the PFGE profiles with an additional 80 kb band in the lower 3 isolates.
Figure 3.
Detail of whole genome maps showing differences between HA-MRSA outbreak isolates obtained from a large medical care center in the Netherlands.
Based on molecular typing (spa-typing, MLVA and PFGE) all isolates were indistinguishable.
Figure 4.
Detail of the WGMs of 16 LA-MRSA isolates originating from unrelated veterinarians showing the discriminatory power of whole genome mapping.
The limited variation obtained by MLVA- and spa-typing is displayed on the right hand side of the WGMs. The blowup of the WGMs displays considerable variation in the SCCmec region.
Figure 5.
Complete WGMs of LA-MRSA isolates obtained from a confirmed transmission event.
The 4 isolates represent 3 household members and 1 isolate originated from a pig on the farm. All isolates were identical in PFGE, spa-typing and MLVA.
Figure 6.
Detail of the WGMs of two veterinarians and their household members showing transmission events.
A and B denote the clusters with highly similar WGMs of isolates obtained from VET45 and his household members (light red block). C denotes the cluster with highly similar WGMs of isolates obtained from VET66 and his household members (blue block). Sampling time-points, sampling sites, spa-type, MLVA-type and PFGE-type are indicated on the right hand side of the maps. The PFGE-type numbers are arbitrary numbers.