Figure 1.
Effects of tannic acid on the growth of L. plantarum WCFS1.
Cells were grown in RPM media supplemented with increasing tannic acid concentrations. Control, ⧫; 0.1 mM tannic acid, □; 0.25 mM tannic acid, ▴; 0.5 mM tannic acid, X. CFU, colony-forming units. Representative curves are shown. Values at each time point are the average of at least 2 replica plates. Standard deviations (not shown) were less than 10%.
Figure 2.
Transmission electron micrographs of lag phase-cells of L. plantarum WCFS1 treated with tannic acid.
Cells were grown for: 4 h in RPM medium lacking tannic acid (A); 16 h (B, C, D, E, F) in RPM medium containing 0.5 mM tannic acid. Scale bar 0.2 µm (A, B, C, F); 0.5 µm (D, E).
Table 1.
Expression of selected L. plantarum WCFS1 genes upon tannic acid challenge.
Figure 3.
Effect of tannic acid on PBPs from L. plantarum WCFS1
PBPs were extracted from L. plantarum WCFS1 cells grown in absence (1) or in presence (2) of 0.5 mM tannic acid and labelled with Bocillin FL. These proteins were separated by SDS-PAGE and detected on the gel by fluorography (see Material and methods). Based on their molecular size PBPs were named according to the PBPs annotated in the complete genome sequence of L. plantarum WCFS1 (see text).