Figure 1.
The current distribution range of the wild population, locations of the 43 nests banded in 2011 and 25 nests selected for this study in the wild as well as the Captive Breeding Center of Crested Ibis.
Photo courtesy of Jiao Jingquan.
Figure 2.
Structure of mtDNA control region and position of primers designed for this study.
TAS = termination associated sequences, CSB = conserved sequence block. The primer positions are scored based on the complete mtDNA sequence of Crested Ibis deposited in GenBank (Accession no. NC_008132).
Figure 3.
Partial sequencing chromatographs and peak distributions of genotyping of representative samples showing length polymorphism and heteroplasmy.
A-27 and A-02 had predominant fragments at 221 bp and 243 bp while their sequencing profiles showed relatively clear chromatograms carrying nine and 11 repeats, respectively. Sample A-30 had at least two predominant peaks at 210 bp and 232 bp whilst its sequencing chromatogram included overlapped signals at an interval of two repeats.
Table 1.
Number of repeats in predominant fragments in 33 captive Crested Ibis.
Figure 4.
Histograms of the distribution of predominant fragments with different numbers of repeat.
(A) 21 wild birds, (B) 31 captive birds, (C) Ten offspring of the mating groups 3, 5 and 6 with their mother carrying a predominant fragment of nine repeats, (D) 12 offspring of the mating group 1, 2 and 7 their mother carrying a predominant fragment of 11 repeats.
Table 2.
Number of repeats in predominant fragments in 27 wild Crested Ibis.
Figure 5.
Pedigree chart represents three generations with 17 maternally-related individuals in family No. 1.
G-1, G-2 and G-3 indicate the generations. Squares stand for males and circles for females. Numbers in brackets right to the squares or circles are the number of repeats in every sample.
Table 3.
Nucleotide variation in mtDNA D-loop sequences of Crested Ibis.