Figure 1.
Seed germination rate of maize hybrid Zong3/87-1 and its parents.
HAI, hours after imbibitions *, represent Significance level p<0.05; ** represent Significance level p<0.01.
Table 1.
The differentially expressed protein spots of additive and nonadditive between maize hybrid and its parental lines.
Figure 2.
Differential protein expression patterns and some some identified protein spots between hybrids and its parents.
A Differential protein expression patterns between hybrids and its parents in maize seed embryos. a) additive expression (spot 7262); b)++: above high parent expression (spot 7228); c)+: high parent expression (spot 7554); d)–: below low parent expression (spot 6686); e) –: low parent expression (spot 2992); f) +/−: partial dominance expression (spot 6547); g)D: different from additivity (midparent value), not belonging to any of the other classes (spot 2728); B. Differential expression patterns of some identified protein spots between hybrids and its parents. DS, dry seed; 24 HAI, 24 hours after imbibitions.
Table 2.
Differentially expressed proteins overlapped in dry and 24 h imbibed embryos.
Figure 3.
Analysis of actin expression during germination of maize dry and 24 h imbibed seeds embryos at the protein level shown by western immunoblotting with monoclonal antibodies MabGpa.
A, The presence of actin isoforms in the maize embryo was analysed by 2-DE. B, Western immunoblotting detected the 42 kDa actin. Equivalent amounts of protein (20 µg) were loaded. C, Protein profile obtained on a 12.5% polyacrylamide gel stained with Coomassie Brilliant Blue. DS, dry seed; 24 HAI, 24 hours after imbibitions.
Figure 4.
The germination phenotypes of the Atact7 mutants and complementation of the Atact7 allele with the maize gene ZmACT2.
A, Actin protein expression in the Columbia, Atact7 mutant and complementation of the Atact7 allele with the maize ZmACT2. The delay in germination is restored in Atact7 mutants by the presence of ZmACT2 as shown for two complemented lines (Atact7&AtACT7P::ZmACT2#4 and #6). Total protein extracts from 48 h germinating seeds was resolved by SDS-PAGE and western immunoblotting detected the actin, hisone H3 as loading control. B, Seeds were stratified at 4°C in the dark for 48 h and incubated at 20°C with MS media for seed germination. C, Seed germination of Arabidopsis thaliana Columbia, Atact7 mutants and complementation of the Atact7 mutant with the maize gene ZmACT2. Germination percentage was counted with time course. Asterisks indicate statistically significant differences compared with the Atact7 mutants. (**Represent Significance level P<0.01).