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Table 1.

Sample sizes at each depth at each of the three collection sites.

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Table 2.

Genotyping statistics for each AFLP selective primer pair.

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Table 3.

Pairwise comparisions of genetic differentiation between depths sampled at Little Cayman Island and Lee Stocking Island using a nested Analysis of Molecular Variance (AMOVA).

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Table 4.

Assignment of randomized samples based upon AFLPOP analysis of band frequencies.

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Table 5.

Assignment of samples based upon AFLPOP analysis of band frequencies at each collection depth.

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Table 6.

Assignment based upon AFLPOP analysis of band frequencies of samples categorized only by depth (LCI, LSI and SS collection sites combined).

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Figure 1.

Plot of standardized coefficients for canonical variables based upon discriminant function analysis of samples classified only by collection sites.

Little Cayman Island –open circles, San Salvador – closed squares, Lee Stocking Island – open squares.

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Figure 2.

Plot of standardized coefficients for canonical variables based upon discriminant function analysis of samples classified only by collection depth.

Shallow (3–10 m) - circles, Medium depth (15–25 m) – open squares, Deep (30–50 m) – closed squares, and Very Deep (60–90) – diamonds

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Table 7.

Band frequencies for AFLP markers used in discriminant function analysis (DFA).

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Table 8.

Band frequencies for AFLP markers used in discriminant function analysis (DFA).

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Figure 3.

Plot of marker frequencies for three markers identified in the FST outlier analysis to exhibit significant deviation from neutral expectations.

The three markers in the Lee Stocking Island samples all showed significantly higher FST compared to neutral expectations suggesting positive selection. The three markers in the Little Cayman Island data set all showed significantly lower FST suggesting balancing selection.

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