Figure 1.
Representative seedlings and adult plants of transgenic petunia.
A. Germination of T3 seeds of line E7H and line E9G on MS medium. B. Growth and development of line E7H and E9G under normal condition. WT was used as control.
Figure 2.
Induced etr1-1 gene expression leads to transgenic plant insensitivity to ethylene.
Seeds of WT and line E7H were planted on 30 μM DEX with or without 20 μM ACC and grown in the dark for 8 days. Quantitative measurements for hypocotyl lengths are shown in panel A. Values represent the means ±SD of at least 20 seedlings from three independent biological replicates. Representative seedlings of wild-type and transgenic line are displayed in panel B.
Figure 3.
Semi-quantitative RT-PCR analysis of transcript levels of induced etr1-1 gene expression.
Petals of transgenic lines E7H and E9G were collected at 0h, 24 h and 48 h after with (+) or without DEX (−) treatments. WT petals and no template (−) were used as negative controls and plasmid DNA (+) was used as a positive control. 26S rRNA was used as an internal control.
Figure 4.
Inducible etr1-1 gene expression extended flower longevity.
Representative flowers either in the presence (+) or absence (−) of DEX were shown in the panel A. Flower longevities with the means ±SD are shown in the panel B. 20 flowers from the wild-type and each transgenic line were used for longevity evaluation.
Figure 5.
Delayed ethylene production by induced etr1-1 gene expression.
Ethylene productions from flowers of transgenic lines with (+) or without DEX (−) were monitored. WT was used as a control and empty cuvettes were used as a reference. Each independent experiment was repeated 3 times. Values represent the means of at least 6 flowers.
Figure 6.
Distributions of GO term identified in comparisons of samples with and without DEX treatment.
Gene ontology analysis of the differential genes in petals with (+) versus without (−) DEX at 24 h (A) and 48 h (B).
Table 1.
Summarized results from GO category based on biological process for differentially expressed genes in comparisons of samples with DEX (+)/without DEX (−) at 24 h.
Table 2.
Summarized results from GO category based on biological process for differentially expressed genes in comparisons of samples with DEX (+)/without DEX (−) at 48 h.
Figure 7.
Expression profiles of genes from samples with (+) or without (−) DEX treatment.
The horizontal axis represented given time points with (+, left panel) or without DEX (-, right panel) treatment, and the vertical axis shows the time series of expression levels for the gene after Log normalised transformation.
Figure 8.
Validation of microarray data by quantitative real-time PCR.
Six genes were selected and their time-course expression profiles were evaluated by quantitative real-time PCR in samples with (+) or without (−) DEX at given time points. (A) Fold changes were obtained from microarray analysis. (B) Fold changes were obtained from qRT-PCR analysis. Relative expression was obtained using 26S rRNA as an internal control. cDNAs were synthesised from three biological replicates.