Table 1.
Zero-, first- and second order Spearman's rho values for correlations between nCB1IR, pEGFR-IR or ErbB2-IR and pAkt-IR.
Figure 1.
Tumour nCB1IR: variation with pAkt-IR.
Panel A shows two tumour cores from the same case (Gleason score 7) showing a large variation in the CB1IR intensity. They had positions 1 and 5 of the tumour series arrowed. The left core was scored 0.75 (50% score 0, 25% score 1, 25% score 2) by one investigator, and 1 (25% score 0, 50% score 1, 25% score 2) by the other. The right core was scored 2.75 by both investigators (50% score 2, 25% score 3, 25% score 4 by one investigator; 25% score 2, 75% score 3 by the other investigator). Panel B and C show the pAkt scores for individual cores in the test (n = 595, Panel B) and validation (n = 297, Panel C) datasets, divided into approximate nCB1IR quadrants. The number of cases in each quadrant is shown in the graph, together with the median and interquartile ranges. *P<0.05, **P<0.01, ***P<0.001 for the comparisons shown, otherwise not significant (Dunn's multiple comparison test following significant (P<0.0001) Kruskal-Wallis test. The spearman's rho for correlations between the core nCB1IR and the core pAkt-IR were 0.29 and 0.28 (both P<0.0001) for the test and validation sets, respectively.
Figure 2.
Scatter plots of cases scored for tumour nCB1IR (abscissae) and pAkt-IR (ordinates) and sorted on the basis of A, Gleason score (GS); B, absence or presence of metastases at diagnosis; C, Ki-67 index; D, tumour grade (LT).
The Ki67 index is a continuous variable ranging from 0–48% in the dataset [34]. The tranches were chosen here for illustrative purposes but represent the bottom 50% (“Ki67 Low”), the 50–75% (“Ki67 intermediate”) and the top 25% (“Ki67 high”). The dotted lines in the figures show the median scores for nCB1IR and pAkt-IR for the dataset.
Figure 3.
Scatter plots and Kaplan-Meier plots for the cases who were followed by expectancy and who had been scored for both nCB1IR and pAkt-IR.
Panel A shows a scatter plot of the individual cases, so that the group names in the other Panels are easier to follow. In the Kaplan-Meier plots shown in Panels B to D, †Pca refers to the number of patients who died as a result of their prostate cancer during the follow-up period. The 2 values are for the log-rank (Mantel-Cox) tests, with the P values shown. Panel B, all cases; Panel C, Gleason score 4–6 cases; Panel D, Gleason score 7–10 cases.
Table 2.
Age, Gleason scores, incidence of metastases at diagnosis and tumour Ki67-IR at diagnosis for the cases divided on the basis of median splits of the tumour nCB1IR and pAkt-IR scores.
Table 3.
Ordinal regression analyses with tumour pAkt-IR and nCB1IR as the independent variables and the Gleason score, %ca or Ki67-index as the dependent variable.
Table 4.
COX proportional-hazards regression analyses for tumour nCB1IR and pAkt-IR for patients with were followed by expectancy.