Figure 1.
Defining developmental and anatomical characters in mature leaves.
[A–E] Morphological performance of CK leaf [A], BD1 leaf [B], BD2 leaf [C], BD3 leaf [D], and BD4 leaf [E]. [F–J] Light micrograph of lateral vein of CK [F], BD1 [G], BD2 [H], BD3 [I], and BD4 [J].
Figure 2.
B concentration of whole leaf.
Columns and bars represented the means and standard error of B concentration respectively (y-axis). Three replicates were used for leaf B concentration measure. DW, dry weight.
Table 1.
Major characteristics of eight libraries.
Table 2.
Differentially expressed genes across all libraries.
Figure 3.
Gene classification based on gene ontology (GO) for differentially expressed genes in BD3 and CK3 libraries of citrus vein.
Only the biological processes were used for GO analysis. The y-axis and x-axis indicated the names of clusters and the ratio of each cluster, respectively.
Figure 4.
Overview of major pathways and their expression profiles during the corky split vein development.
[A] Overview of major pathways in corky split vein caused by B deficiency. [B–G] Expression profiles of the DEGs involved in cell cycle [B], DNA replication [C] lignin biosynthesis [D], cytokinin signal transduction [E], vascular development [F], and photosynthesis [G] pathways from BD1 phase to BD4 phase. Pathways with transcripts that were up-regulated or down-regulated were indicated with + or −. CTK, cytokinin. DEGs, differentially expressed genes.
Table 3.
Selected genes with altered expression across all libraries.
Figure 5.
qRT-PCR confirmation of differentially expressed genes from digital gene expression analysis.
[A–I] The transcript levels from qRT-PCR and digital gene expression analysis of differentially expressed genes: MCM3 [A], MCM5 [B], POLD2 [C], WOX4 [D], VND7 [E], MYB85 [F], CAD [G], CCR [H], and PETE1 [I]. Relative expression levels were calculated by qRT-PCR with actin as a standard. Columns and bars represented the means and standard error of relative expression levels (n = 3) respectively (Y axis at left). Lines represented the means of RPKM value calculated by digital gene expression analysis (Y axis at right). The x-axis indicated the phases of vein development. RPKM, Reads Per kb per Million reads.