Figure 1.
Mycetoma of the foot from Nocardia brasiliensis showing the characteristic triad of tumefaction, fistulae and microcolonies.
Central image, an X-ray analysis of the ankle and foot region showing the severe destruction of bones. Right image, a microcolony of N. brasiliensis stained with PAS surrounded by a PMN infiltrate.
Figure 2.
Physical map of the N. brasiliensis chromosome and gene clusters for putative virulence genes.
The outer scale is numbered in megabases starting from the dnaA gene. The outermost two circles represent the position and strand direction of putative virulence genes and transposases. Circles 3, 4, and 5 represent a BLAST comparison with the complete genome of Nocardia farcinica IFM 10152 (NC_6361.1, red), Rhodococcus equi 103S (NC_014659.1, cyan) and Mycobacterium tuberculosis H37Rv (NC_000962.2, yellow), respectively. Circle 6, GC content; circle 7, GC bias. In magenta, we show the fragment with different G+C% (between nucleotide 5,126,000 and nucleotide 5,800,000).
Table 1.
Comparison of genomic features of Nocardia brasiliensis and other bacteria.
Figure 3.
Syntenic dot plot of N. brasiliensis ATCC 700358 genome against Nocardia farcinica IFM10152 (a), Nocardia cyriacigeorgica GUH-2 (b), Rhodococcus equi 103S, Amycolatopsis mediterranei U32, Streptomyces coelicolor A(2), and Mycobacterium tuberculosis H37Rv genomes.
Dots represent a reciprocal best match by BLASTP comparison. The x-axis corresponds to the N. brasiliensis genome plotted against the rest of the genomes (y-axis). Inclination to the right corresponds to ORFs in same direction. An inclination to the left corresponds to an opposite direction. The highest homology in the Nocardia species was found at about the dnaA site. In each case, genome coverage was 30, 30, 11, 7, 5 and 4%.
Table 2.
Distribution of putative virulence factors among actinomycetes with complete genome sequence.
Table 3.
Distribution of important genome features among actinomycetes with complete genome sequence.