Figure 1.
cDNA-AFLP analysis of transcripts in response to MeJA treatment in G. lucidum.
cDNA-AFLP silver-stained polyacrylamide gels with 9 primer combinations (PC) amplifying differentially expressed genes in G. lucidum treated with 0, 50 and 200 µM MeJA, respectively. The combinations of primers used are indicated according to the codes reported in Table S4. The molecular weight marker sizes are indicated on both sides. Arrows indicate some of the differentially expressed transcript-derived fragments.
Table 1.
Classification of TDFs from the cDNA-AFLP result in functional categories.
Table 2.
Transcript derived fragments (TDFs) from G. lucidum with homologies to other known protein.
Figure 2.
Percentages of 90 known functional TDFs with functional categories.
A. The functional classification of transcript-derived fragments (90 TDFs) from G. lucidum in response to MeJA. The percentages of differentially expressed genes in the 90 known functional sequences assigned to various functional categories. B. Ninety TDFs in biological function categories showing differential expression patterns in G. lucidum. The percentages of different functional categories of up- and down-regulated sequences in the 90 known functional sequences were significantly different (P<0.05).
Figure 3.
qRT-PCR analysis of 25 selected TDFs in G. lucidum.
Expression of 25 selected genes treated with 0, 50 and 200 µM MeJA, respectively. aao (TDF096) aryl-alcohol oxidase, nbp (TDF009) nucleotide binding protein, cdc (TDF122) cell division control protein, cal (TDF375) calcium transporting ATPase, cat (TDF129) catalase, vmp (TDF264) vacuolar membrane protein, hd (TDF293) histone deacetylase, gls (TDF080) glucosidase I, hk (TDF051) histidine kinase, fum (TDF195) fumarase, ksr (TDF256) ERG27-3-keto sterol reductase, apk (TDF040) cAMP-dependent protein kinase, nuc (TDF058) IMP-specific 5′-nucleotidase 1, gfd (TDF291) glutathione-dependent formaldehyde dehydrogenase, pyr (TDF323) pyruvate kinase, mob (TDF052) protein kinase activator, pco (TDF243) pyruvate carboxylase, guf (TDF078) glycerol uptake facilitator, prp (TDF042) pre-mRNA splicing factor, vac (TDF360) vacuole protein, aact (TDF113) acetyl-CoA acetyltransferase, ndd (TDF015) NAD-dependent deacetylase, mapk (TDF013) CMGC/MAPK/JNK protein kinase, rho (TDF165) small monomeric GTPase, cyt (TDF047) cytochrome b2. All samples were examined in triplicate. For all genes represented in this figure, the P value was <0.05 or 0.01 (*p<0.05 and **p<0.01).
Figure 4.
The expression of TDF genes in different development stages.
A. Venn diagrams depicting the genes expressed across MeJA-induction and the different developmental stages. Data are derived from Table S3. B. The transcript levels of TDF genes under the developmental stages of G. lucidum. The X axis shows the abbreviation of genes. The full name of each gene is in the Figure 3 legends. All samples were examined in triplicate. For all genes represented in this figure, the P value was <0.01.
Figure 5.
Schematic pathway predicting the role of MeJA induced genes in G. lucidum.
Integrated pathway map shows the role of MeJA-induced genes involved in GA biosynthesis, primary metabolism, signaling regulation and transcriptional regulation. Dashed lines indicate the probable pathway involved in GA biosynthesis. Solid lines indicate the result supported by cDNA-AFLP and real time PCR. The ESTs from cDNA-AFLP results are indicated in stars.