Table 1.
Strains, plasmids and primers used.
Table 2.
Transformation efficiencies and transposition frequencies.
Figure 1.
Confirmation of transposon insertion events into B. breve UCC2003 and B. breve NCFB2258 genome by Southern hybridization.
Blots of twenty-six randomly selected mutants of B. breve UCC2003 (Panel I, two blots) and sixteen randomly selected mutants of B. breve NCFB2258 (Panel II, single blot) are shown. Lanes labeled from A to R correspond to mutants whose insertion site was subsequently sequenced as indicated in Table 3.
Table 3.
Mapping transposon insertion sites in a random selection of transposon insertion mutants.
Figure 2.
Growth profiles of B. breve UCC2003 and derived transposon mutant strains, 101C6 (I), 181D10 (II) on lactose, galactose and ribose; and 164B7 (III) on lactose, pullulan and ribose.
Presented data are average of duplicate independent growth experiments.
Figure 3.
Genomic position and surrounding regions of the insertion site of a number of transposon insertion mutants that were isolated based on their inability to grow on one or more carbohydrates.
The diagram was drawn to scale using B. breve UCC2003 genome sequence information. White open arrowheads represent the location of the transposon with the specific location indicated as the genome coordinate based on accession number CP000303. Red arrows represent transposon-disrupted open reading frames. Grey arrows represent flanking open reading frames. Lollipops indicate transcriptional terminators predicted by ARNold Web server (http://rna.igmors.u-psud.fr/toolbox/arnold/).