Figure 1.
Cd hyperaccumulation in S. alfredii Hance (HE).
(A) Growth of HE after exposure to 0 µM (Cont) or 100 µM Cd2+ (Cd) for 8 days. (B) Cd content (mg kg−1 dry weight) in shoots and roots of HE. Bars depict SE (n = 3).
Figure 2.
Gene Ontology (GO) analysis of S. alfredii Hance (HE) contigs.
GO distribution of (A) cellular component, (B) biological process and (C) molecular function.
Figure 3.
Proportion of S. alfredii Hance (HE) contigs in different functional categories.
KEGG (Kyoto Encyclopedia of Genes and Genomes) Orthology Groups (KOG) analysis was applied to the assembled contigs.
Figure 4.
Homologous protein comparisons of S. alfredii Hance (HE) to other sequenced model plants.
(A) Distribution of S. alfredii homologous proteins identified in other plants using several BlastP E-value cutoffs. (B) Comparison of the number of homologs identified between S. alfredii and other plant proteomes with BlastP E-value cutoff of 1E-30. The number of contigs in each species is displayed in the same color as the species name.
Table 1.
Expression levels of Cd up-regulated contigs in HE shoots.
Figure 5.
Comparison of gene expression in S. alfredii Hance hyper-accumulation ecotype (HE) and non-hyperaccumulation ecotype (NHE).
(A) Transcriptional responses of two ecotypes to Cd exposure. Fold change of expression is the relative gene expression level in Cd treatment divided by the value in the control. (B) Relative gene expression levels of two ecotypes under normal growth condition. The relative gene expression is the expression level normalized to a constitutively expressed gene Actin. P-type metal ATPase HMA4: Sa_Contig11685; Zinc transporter ZIP1: Sa_Contig10290; Metal tolerance protein MTP3: Sa_Contig47062. The contig numbers for other genes are listed in Table 1. P values are indicated as follows: *P<0.05; **P<0.01; ***P<0.001. Bars depict SE (n = 3).