Table 1.
Number of bank voles captured during the study, PUUV prevalence and proportions of A and B genogroups and reassortants at Konnevesi.
Figure 1.
Phylogenetic trees (Neighbor-Joining) calculated for the S, M and L segment sequences of PUUV, and contings of the three segment sequences: (A) partial S (nt 631–1085); (B) partial M (nt 2162–2613); (C) partial L (nt 505–1040); (D) a contig of the three genome segments omitting reassortant variants, and (E) a contig of the three genome segments including reassortant variants. A maximum clade credibility tree with an arbitrary root is shown with mean branch lengths (substitutions per site), and non-parametric bootstrap percentages are shown for each node. DOBV, ANDV, SNV and TULV were used as outgroups and omitted for the graphical representation.
Table 2.
Genetic diversity of PUUV variants at Konnevesi.
Figure 2.
Genetic relationships between (A) S-segment, (B) M-segment and (C) L-segment genotypes. Relationships were constructed using Network analysis and the MJ algorithm.
Genotypes of group A are represented by red circles and genotypes of group B by blue circles. The size of the circle is proportional to the number of representatives of each genotype; and numbers are shown inside. Yellow circles are median vectors suggested by Network. The numbers on the link-lines show the number of mutations between genotypes; the length of link-lines is not proportional to the number of mutations.
Table 3.
Genetic variants of PUUV observed in the Konnevesi bank vole population.
Figure 3.
PUUV segment genotypes circulating in the bank vole population at Konnevesi from 2005 to 2009.
Relationships between S-, M- and L-segment genotypes was constructed using Network. Genotypes of group A are represented by red circles and genotypes of group B by blue circles. The size of the circles is proportional to the number of representatives of each genotype; and numbers are shown inside. Most abundant circulating genotypes (copious) are designated in red and blue, for the A and B genogroups, respectively. Sporadic genotypes, detected only once, are shown in black and sporadic genotypes observed through several seasons are shown in a square. Yellow circles are median vectors suggested by Network. The numbers on the link-lines show the number of mutations between genotypes; the length of link-lines is not proportional to the number of mutations. Note that the trapping effort was increased from 2007 thus numbers from 2005 are not directly comparable to those of 2007–09.
Table 4.
PUUV variants repeatedly detected in the bank vole population.
Table 5.
Proportion of copious segment genotypes that composed the PUUV variants detected during the five-year study period.
Table 6.
PUUV Reassortants.