Figure 1.
Characterization of human PH patients profiles.
A. The PAM, PAS and PAD were determined by catheterization as described in material and methods. B. Determination of profiles for pulmonary vascular resistance (PVR), C. Right atrial pressure (RA), D. Right ventricular systolic pressure (RVSP), E. Right ventricular internal diameter (RVID) and F. Ejection fraction (EF). Data represent means ± SEM. N = 8 for control, 15 for moderate PH and 17 for severe PH group. *denotes p<0.001 and ** denotes p<0.05 compared to the healthy subjects, n.s. is non-significant.
Table 1.
The one-way ANOVA was performed to determine the significance level among the groups.
Figure 2.
Heat map showing the expression of dysregulated miRNAs in control and PH human subjects.
MiRNA microarray data was prepared using data from the miRNA microarray performed using samples from PH human patients and compared with healthy controls. The signal difference (log2) is plotted versus the level of statistical significance (−log10 p-value). Color and spot diameter depicted information about mean expression level and percent detected, respectively, in static plots.
Figure 3.
Real time-PCR based validation of downregulated miRNAs in the buffy coat of healthy human subjects vs. PH patients.
A. Expression of miR-1, miR-26a and miR-29c B. Expression of miR-34b, miR-451 and miR-1246. Data represent means ± SEM. N = 16 for control, 15 for moderate PH and 17 for severe PH group. *denotes p<0.05 and ** denotes p<0.01 compared to the healthy subjects.
Figure 4.
Real time-PCR based validation of upregulated miRNAs in the buffy coat of healthy human subjects vs. PH patients.
A. Expression of miR-21 and miR-23a, B. Expression of miR-30b and miR-130a, C. Expression of miR-133b and miR-191, D. Expression of miR-204 and miR-208b. Data represent means ± SEM. N = 16 for control, 15 for moderate PH and 17 for severe PH group. *denotes p<0.05 and ** denotes p<0.01 compared to the healthy subjects.
Figure 5.
Effect of gender on dysregulated miRNAs miRNAs were detected in the validation cohort of PH by RT-PCR and was normalized using has-U6 housekeeping gene.
Data represent means ± SEM, n = 12–15 for female PH subjects and n = 10–12 for male PH subjects.