Figure 1.
Laser capture microdissection of nodule cells.
Panels represent 8 µM thick longitudinal sections of 3-week old Medicago nodules before capture (a,d,g,j,m), after capture (b,e,h,k,n) and captured/isolated cells (c,f,i,l,o). Cells/tissues were isolated from the meristem (m; a–c), distal infection zone (diz; d–f), proximal infection zone(piz; g–i), infected cells (ic; j–l) and uninfected cells (uic; m–o) from the fixation zone.
Figure 2.
(a,b) In situ localization of MtENOD12 (antisense probe) in the infection zone of longitudinal sections of 14-day-old Medicago nodules, representing brightfield (a; signal appears as black dots) and epipolarization images (b). (c,d) Promoter-GUS analysis of Medicago ROP GTPase (Mtr.35940.1.S1_at, Mtr.15539.1.S1_at), showing β-glucoronidase (GUS) activity in the nodule meristem. (e,f) Promoter-GUS analysis of MtENOD8.2, showing β-glucoronidase activity in the non-infected cells of the nodule as well as in the nodule parenchyma.
Table 1.
Number of genes showing cell/tissue specific or enriched expression.
Table 2.
Selection of genes with known expression profiles used for validation.