Figure 1.
Fractionation of IgG subclasses from human IVIG by FPLC.
IgG3 protein was present in the initial flow-through, as indicated. Upon application of a gradually diminishing pH gradient, IgG2 was eluted, followed by IgG1. mAU, milli-Absorption Unit at 280 nm.
Table 1.
Fractionation and purification of human IgG subclasses.
Figure 2.
The contribution of each human IgG subclass to EV71 neutralization.
Neutralization tests of IgG subclasses were performed using in RD cells for at least 3 times. Error bars represent standard deviation (SD), and statistical analysis was performed by using GraphPad Prism v5.0 software by Student’s t test (unpaired, two-tailed). ***, p<0.005 versus parent IVIG lot.
Figure 3.
ADE profile of each human IgG subclass against EV71.
Ten-fold dilutions of each IgG subclass and parent IVIG was incubated with EV71 strain AH08/06 at an MOI of 1 before incubation with human THP-1 cells, and the ADE potential was represented as fold increase of progeny viral titer compared with the PBS group.