Figure 1.
Distribution of R. soongorica in northern China.
The blue shadow indicates the Reaumuria distribution in China and neighboring districts. The black shadow indicates the Reaumuria distribution in North Africa and Central Asia.
Table 1.
Summary of de novo sequence assembly for Reaumuria soongorica.
Figure 2.
Characteristics of similarity search of unigenes against Nr databases.
(A) The E-value distribution of Nr annotation results. (B) The similarity distribution of Nr annotation results. (C) The species distribution of Nr annotation results. (D) The phylogenetic relationship of Reaumuria soongorica and Nr species. Phylogenetic tree constructed by the Neighbor-Joining method for the internal transcribed spacer (ITS) sequences. Physcomitrella patens was included as an out-group. Neighbor-Joining was consensus tree used 1000 bootstrap replicates. The number represents the percentage of bootstrap values.
Table 2.
Summary of sequence annotation for Reaumuria soongorica.
Figure 3.
Gene Ontology classification of assembled unigenes.
Total 29,666 unigenes were categorized into three main categories: Biological process (81,460, 40.21%), Cellular component (83,650, 41.29%) and Molecular function (37,497, 18.51%).
Figure 4.
Total 14,987 unigenes were assigned to 25 COG classifications. The largest cluster was for general function prediction only (4,564, 15.99%), followed by transcription (2,455, 8.60%), replication, recombination and repair (2,335, 8.18%), translation, ribosomal structure and biogenesis (2,243, 7.86%), posttranslational modification, protein turnover, and chaperones (2,157, 7.56%), carbohydrate transport and metabolism (1,795, 6.29%) and signal transduction mechanisms (1,788, 6.27%).
Figure 5.
Boxplot distribution of unigenes in annotated and un-annotated unigenes.
(A) Length distribution inferred with log(Length). (B) Expression level inferred by log(RPKM) value. Both show significant in ANOVA tests p = 2.2e-16.
Figure 6.
Boxplot distribution of C3 and C4 unigenes.
(A) Length distribution inferred with log(Length). (B) Expression level inferred by log(RPKM) value.