Table 1.
The number of samples and the Symbiodinium ITS2 DGGE profiles for each coral species from each site used in this study.
Figure 1.
A representative of each Symbiodinium ITS2 DGGE profile from the seven coral species investigated.
The Symbiodinium profiles from the species Acropora millepora (Am), Pocillopora damicornis (Pd), Seriatopora hystrix (Sh), Porites cylindrica (Pc), massive Porites sp. (Pm), Galaxea fascicularis (Gf) and Favites pentagona (Fp) are shown at the top of each DGGE column. Each of the 13 dominant bands (I-XIII), which characterise the profiles, are also indicated. Bands VI and VII are labelled twice as they appear to co-migrate, however sequence data differentiates them.
Figure 2.
Symbiodinium ITS2 haplotype networks of the 13 dominant bands identified in this study.
Parsimony networks of Clade D (A) and Clade C (B) Symbiodinium ITS2 haplotypes from dominant bands identified in this study. Coral species are shown in different colours and Roman numerals indicate dominant band numbers. Previously published sequences are also indicated (1–4) along with their Genbank accession number. Each node represents a base pair change and indel lengths are shown by the boxed numbers along branches. Bands III to XI and XIII represent clade C types, while bands I, II and XII represent clade D type Symbiodinium. Pies indicate the presence of Symbiodinium types in multiple coral species and are not indicative of frequency.
Figure 3.
The frequency of different Symbiodinium ITS2 profiles between sites and CO2 exposures.
The different Symbiodinium ITS2 profiles from the corals Acropora millepora, Pocillopora damicornis, Seriatopora hystrix, Porites cylindrica, massive Porites sp. and Galaxea fascicularis found at the three sites, each with High (Hi) and control (Lo) CO2 exposures. Each colour corresponds to one of the 14 unique Symbiodinium ITS2 profiles found in these coral species. Colours common between sites or coral species indicate synonymous Symbiodinium profiles (See also Table S1). The different colour schemes represent clade C (shades of green, yellow and brown), D (shade of grey and blue) and mixed (pink) Symbiodinium.