Figure 1.
A hypomelanistic endemic endangered Puerto Rican Boa (Epicrates inornatus) consuming a bat (Monophyllus redmani), Mata de Plátano Cave, Arecibo municipality.
Photo by ARPR.
Figure 2.
Map of Puerto Rico overlaid with the karst region (brown) and mtDNA haplogroups found in the Puerto Rican Boa, mapped by sampling locality on the island.
Sampling locations with a single individual are labeled with a “1.” Note that no distinct phylogeographic signal is found, and that Haplogroup I appears restricted to a single population.
Table 1.
Locality information, number of samples, and sampling type(s) for cave, surface, and all other populations of Epicrates inornatus sampled from Puerto Rico.
Figure 3.
Map of Puerto Rico, where municipalities with samples of Epicrates inornatus are individually colored.
A haplotype network is shown above the map, with each haplotype color-coded by the proportion of individuals from each municipality represented in the haplotype. Haplotype circles are sized proportionally to the number of individuals represented in each haplotype, where the smallest circles represent one individual and the largest represents 13 individuals. Line segment lengths are arbitrary, and the smallest black circles represent un-sampled mutational steps. Three haplogroups are recovered and separated into gray boxes. Two cave (Agrodel [Hatillo], and Mata de Plátano [Arecibo]) and two surface (Río Encantado [Ciales], and Dorado Beach [Dorado]) populations are labeled both on the map and on the haplotype network.
Figure 4.
Plots of genetic diversity for cave and surface populations of Puerto Rican boas.
A) mtDNA and nucDNA estimates for each cave and surface population. The parameters are haplotype diversity (h), observed heterozygosity (HO), and allelic richness (A). B) Genotypic differentiation among populations visualized in multivariate space using principal components rotations. Inset: P-values for Fisher’s tests for genetic differentiation between populations.
Table 2.
Genetic diversity and test statistics of selective neutrality with populations of Epicrates inornatus for the concatenated mitochondrial dataset.
Table 3.
Results of analysis of molecular variance (AMOVA) for various groupings of Epicrates inornatus for both mtDNA and microsatellite data.
Table 4.
Pairwise genetic divergence between sampling locations (cave and surface populations only).
Table 5.
Microsatellite loci used to genotype samples in this study.
Table 6.
Sample sizes and summary statistics of microsatellite gene diversity (SE) for the two cave and two surface populations.