Figure 1.
Schematic diagram of the sesame populations used for seed coat color trait analysis.
Populations derived from six generations of P1, F1, P2, F2, BC1, BC2, were investigated to determine the genetic basis of the seed coat color trait. 260 lines of an F2 mapping population were screened to construct a genetic linkage map. 260 lines of an F2 mapping population and their F3 families were used for QTL determination.
Table 1.
Distribution of RGB values for seed coat color in six genetic populations of sesame.
Table 2.
AIC and maximum log likelihood values of 24 genetic models estimated using an iterated ECM (IECM) algorithm.
Table 3.
Estimation of 1st order genetic parameters for seed color trait.
Table 4.
Estimation of 2nd order genetic parameters for seed color trait.
Figure 2.
Distribution of QTLs for sesame seed color trait on our seame high-density genetic linkage map.
653 marker loci were distributed across the 14 linkage groups of our high-density genetic linkage map at an LOD threshold of 6.0. The cM distance of markers is shown on the left side of each LG. The name, amplicant length (bp) and band number of each marker are shown on the right side of each linkage group. An LOD peak value of >2.5 was considered to indicated a significant QTL interval. The four QTLs identified using two programs are designated as follows: winQTL-1, winQTL-2 and winQTL-3 represent the QTL loci from the F2 population and F3 families (Yuanyang) and F3 families (Pingyu), respectively using the winQTLCart program. QTLnet-1, QTLnet-2 and QTLnet-3 represent the QTL loci from the F2 and F3 family (Yuanyang) and F3 family (Pingyu), respectively, using networks program.
Table 5.
QTL analysis of seed color trait in F2 and F3 families using winQTLCart program.
Table 6.
QTL analysis of seed color trait in F2 and F3 families using QTLnetworks 2.0 program.
Table 7.
ANOVA of seed color trait in the F3 families.