Figure 1.
Mean biomass growth [mg DW L−1] of Picochlorum atomus at 2, 8, 11, 18, 28 and 36 ppt determined using% transmittance at 750 nm.
Arrow: indicates the addition of nutrients. Active growth was divided into 3 phases (I–III) based on log-transformed data for determination of specific growth rates [µ]. n = 3. Standard error is shown. DW: dry weight.
Table 1.
Effect of salinity on specific growth rates [μ], divisions per day and generation times [days] of Picochlorum atomus.
Table 2.
Effect of salinity on volumetric biomass productivities of Picochlorum atomus during growth phases I and II, and overall from days 2–18.
Figure 2.
Effect of salinity on nitrate assimilation [mg L−1] of Picochlorum atomus.
n = 3. Standard error is shown. Arrow: indicates measurements taken after nitrate and phosphate replenishment.
Figure 3.
Effect of salinity on media nitrite dynamics [mg L−1] of Picochlorum atomus.
n = 3. Standard error is shown. Arrow: indicates measurements taken after nitrate and phosphate replenishment.
Figure 4.
Total daily net nitrogen [N] uptake [mg L−1] of Picochlorum atomus.
Average total nitrogen consumption is shown for salinities of 2, 8, 18, 28 and 36 ppt, while nitrogen consumption of cultures at 11 ppt is plotted individually to highlight the effect of 11 ppt. n = 3. Standard error is shown.
Figure 5.
Effect of salinity on phosphate assimilation [mg L−1] of Picochlorum atomus.
n = 3. Standard error is shown. Arrow: indicates measurements taken after nitrate and phosphate replenishment.
Figure 6.
Effect of nutrient availability and salinity on total lipid and fatty acid content.
Total lipid and FAME content under nutrient replete conditions (A) and nutrient deplete conditions (B). Grey bars: total lipid, white bars: total FAME. n = 3. Standard error is shown. Statistically significant differences are indicated by different letters. Prime letters are used for total FAME.
Table 3.
Total FAME productivities [mg L−1 day−1] of Picochlorum atomus from nutrient replete to deplete conditions.
Table 4.
Effect of salinity and culture nutrient status (replete/deplete) on fatty acid profiles (proportion [%] of total FAME) of Picochlorum atomus.
Figure 7.
Effect of salinity and culture nutrient status (replete/deplete) on mean carbohydrate content [mg glucose g−1 DW] of Picochlorum atomus.
n = 3. Standard error is shown. Different letters show statistical significance.
Figure 8.
Effect of salinity and culture nutrient status (replete/deplete) on mean protein content [mg protein g−1 DW] of Picochlorum atomus.
n = 3. Standard error is shown. Different letters show statistical significance.
Figure 9.
Effect of salinity (11, 18, 28 and 36 ppt) on the proportion [%] of Pseudanabaena limnetica in Picochlorum atomus cultures.
n = 3. Standard error is shown.
Table 5.
Comparison of growth data and reported ranges in this study with growth data obtained for Picochlorum spp./Nannochloris spp. under similar* and deviating cultivation conditions.