Figure 1.
Co-localization of the GR and α-catenin to the plasma membrane of keratinocytes.
Immunofluorescent staining of primary human keratinocytes incubated in the absence or presence of DEX shows co-localization of GR and α-catenin to the plasma membrane. Green fluorescence indicates presence of GR (A); red alpha-catenin (B) and yellow merge (C). Arrows point to the membranous staining. Scale bar 15 µm.
Figure 2.
GR is bound to α-catenin in the plasma membrane of keratinocytes.
(A) Cell fractionation was performed on primary human keratinocytes following 30 min treatment with Dex or vehicle (V). The purity of the fractions was confirmed using: histone H3 or IkBα specific antibodies. H3 was found only in nuclear fractions whereas IkB α was found only in cytoplasmic fractions. (B) Immunoprecipitations following a treatment of keratinocytes with Dex or vehicle were performed with an antibody against α-catenin. The immunoprecipitation was then immunoblotted with GR. (C) Conversely, immunoprecipitation was performed with an antibody against GR. The immunoprecipitation was then immunoblotted with α-catenin. (D) Immunoprecipitations following a treatment of human epidermis with vehicle or Dex was performed with an antibody against α–catenin, and immunoblotted with GR. Normal IgG was used as a control.