Figure 1.
Flow cytometry dot plot of activation markers on CD8+ T cells.
Representative dot plot depicting the use of CD38 and HLADR markers to define activated CD8+ T cell populations. The red numbers in each of corner represent the percentage of CD8+ T cells considered to have each combination of the CD38 and HLA-DR markers. For example, 60.6% of CD8+ T-cells in this plot were classified as being positive for both CD38 and HLA-DR. A similar approach was used with CD4+ T cells.
Table 1.
Baseline descriptive statistics.
Figure 2.
T cell activation as function of diurnal cortisol.
The scatter plots show CD8 and CD4 activation as a function of waking cortisol or diurnal cortisol slope. The slope presented in the graphs is the multivariate regression coefficient for T-cell activation as a function of cortisol measures, adjusted for age, viral load, and CD4 count. Figure 2A shows CD38 mean fluorescent intensity (MFI) on CD8+ T cell in relationship to waking cortisol. Figure 2B shows CD38 mean fluorescent intensity (MFI) on CD4+ T cell in relationship to waking cortisol. Figure 2C shows CD38 mean fluorescent intensity (MFI) on CD8+ T cell in relationship to diurnal cortisol slope. Figure 2D shows CD38 mean fluorescent intensity (MFI) on CD4+ T cell in relationship to diurnal cortisol slope. * = p<.05. ** = p≤.001.
Table 2.
Correlations of T cell activation measures with cortisol measuresa.
Table 3.
Correlations of T cell percent antigen expression with cortisol measuresa.