Table 1.
Summary of patient, isolate and clinical information.
Figure 1.
Genotypic analysis of M. abscessus spp. isolates from persistently infected patients.
Dendrogram constructed using BioNumerics showing the genetic relatedness among ERIC-PCR profiles of 1 representative isolates from each of the 12 patients.
Figure 2.
Phylogenetic reconstruction (MLST) resolves the species designation of each M. abscessus spp. isolate.
Neighbour-joining tree of 13 clinical isolates and type strains M. abscessus NCTC 13031, M. bolletii DSM 45149 and M. massiliense DSM 45103 based on four concatenated MLST gene sequences (argH, glpK, gnd and murC). Colour coding indicates M. abscessus sensu stricto (red), M. bolletii (blue) and M. massiliense (green)-associated clades. Bootstrap support for significant branches is indicated, and branch lengths are representative of substitutions per site.
Figure 3.
Evolutionary genomic analysis of M. bolletii during a 12 year chronic infection of a single CF patient.
(A) Schematic representation of the timescale of infection including isolation dates of smooth colony phenotype (circles), rough colony phenotype (triangles), and sequenced isolates (filled symbols), respectively. Approximate time periods of antibiotic treatment are indicated by arrows. (B) Neighbor-joining phylogenetic tree of M. bolletii isolates from a single patient based on core genome SNPs.
Table 2.
Isolates from patient 1 selected for whole genome sequencing.
Table 3.
Genome variation among sequential M. bolletii isolates from a single patient.