Table 1.
Description of all included studies including differences by endotoxin strain, mouse strain, exposure protocol, and microarray platform used.
Figure 1.
Comparison of 3 identified studies of murine inhaled endotoxin exposure used to generate gene signature.
1a. Common intersect of differentially regulated genes (endotoxin vs. control exposure) identifies a gene signature for endotoxin exposure. 1b. Common intersect of arrays used in each studies represents a background distribution to identify the statistical significance of the gene signature. 1c. 2-way hypergeometric tests to identify statistical significance of gene signature.
Table 2.
Mouse genome identifier (MGI) gene symbols for all genes identified in the 101 gene signature.
Table 3.
Identifying other studies with similar expression of 101 gene signature in the context of the public corpus of gene expression studies using NextBio.
Figure 2.
Comparison of all 4 identified studies at the pathway level.
Pathway enrichment calculated using hypergeometric tests with all known pathways present in Netpath, Wikipathways, Kegg, and Reactome, with Cytoscape for visualization. 2a. Lai et al, 5 day exposure. 2b. Lai et al, 8 week exposure. 2c. Meng et al, 3 week exposure. 2d. Brass et al, 1 week exposure.
Figure 3.
Consensus clustering of air, endotoxin, smoke, and endotoxin plus smoke exposed mice using endotoxin gene signature and random gene signature.
The endotoxin gene signature accurately clusters the different exposure groups 99.97% of the time as compared to a randomly chosen gene signature which accurately clusters the different exposure groups 78.15% of the time.
Table 4.
Description of exposure protocol used in comparing endotoxin, cigarette smoke, and endotoxin plus cigarette smoke exposed mice.