Table 1.
SSR genotypes of species within the genera Chrysanthemum, Leucanthemella, Ajania, Artemisia, Opisthopappus, Pyrethrum, Tanacetum and Crossostephium.
Figure 1.
The distribution of contig and unigene sequence lengths.
Table 2.
Summary of sequencing output statistics.
Figure 2.
The distribution of E-value, sequence similarity, and species of data base hits with the C. nankingense unigenes.
Figure 3.
Functional classification of the C. nankingense unigenes according to COG criteria.
Figure 4.
The distribution of C. nankingense unigenes among the GO functional classes.
From left to right, “Biological process” (shown in blue): biological adhesion, biological regulation, cell killing, cellular component organization or biogenesis, cellular process, death, developmental process, establishment of localization, growth, immune system process, localization, locomotion, metabolic process, multi-organism process, multicellular organismal process, negative regulation of biological process, pigmentation, positive regulation of biological process, regulation of biological process, reproduction, reproductive process, response to stimulus, rhythmic process, signaling, viral reproduction; “Cellular components” (shown in red): cell, cell junction, cell part, extracellular region, extracellular region part, macromolecular complex, membrane-enclosed lumen, organelle, organelle part, virion; “Molecular function” (shown in green): antioxidant activity, binding, catalytic activity, enzyme regulator activity, molecular transducer activity, protein binding transcription factor activity, receptor activity, transporter activity.
Figure 5.
Frequencies of the various repeat motifs present in the C. nankingense EST-SSRs.
Figure 6.
SSR allelic variation among 42 commercial C. morifolium cultivars.
(A) Single product, non-polymorphic amplicon(primer #214), (B) Two product, polymorphic amplicon (primer #86), (C) Multiple product, highly polymorphic amplicon (primer #245). Lanes 1–42: cv. ‘Zhongshanzigui’, cv. ‘Zhongshanjingui’, cv. ‘Zhongshanhongying’, cv. ‘Zhongshanaihuang’, cv. ‘Zhongshanzihe’, cv. ‘Zhongshanchengguang’, cv. ‘Zhongshanhongfeng’, cv. ‘Zhongshanhuangtuogui’, cv. ‘Zhongshanguohuang’, cv. ‘Zhongshanbailu’, cv. ‘Zhongshanfenzhuang’, cv. ‘Zhongshanzilian’, cv. Zhongshanfendai’, cv. ‘Zhongshanqueyu’, cv. ‘Zhongshanhongxia’, cv. ‘Zhongshanhuangying’, cv. ‘Zhongshanhuangyu’, cv. ‘Zhongshanjinyu’, cv. ‘Zhongshanyinxing’, cv. ‘Zhongshanzaobai’, cv. ‘Zhongshanzixing’, cv. ‘Zhongshanziyu’, cv. ‘Zhongshanfengui’, cv. ‘Zhongshanjinzhen’, cv. ‘Zhongshanxuegui’, cv. ‘Zhongshanzuirong’, cv. ‘Jinlingaihuang’, cv. ‘Jinlingbaifeng’, cv. ‘Jinlingbaixue’, cv. ‘Jinlingbaiyu’, cv. ‘Jinlingbaohui’, cv. ‘Jinlingbaoxia’, cv. ‘Jinlingchixin’, cv. ‘Jinlingchunse’, cv. ‘Jinlingfanxing’, cv. ‘Jinlingfendai’, cv. ‘Jinlingguofen’, cv. ‘Jinlingguohong’, cv. ‘Jinlingguohuang’, cv. ‘Jinlingguozi’, cv. ‘Jinlinghonghe’, cv. ‘Jinlinghongpao’.
Figure 7.
UPGMA-based phylogeny of Chrysanthemum spp. and species belonging to closely related genera.
The tree was derived from genotype data from 20 EST-SSR loci. (A–F) Six clades were recognized.