Table 1.
P. aeruginosa strains and plasmids used in this study.
Table 2.
Primers used in this study.
Figure 1.
Inactivation of PA0756-0757, PA2070 or PA5033 does not affect biofilm formation.
A. Air-liquid interface assay of biofilm formation of P. aeruginosa PA14 wild type and its deletion mutants, ΔPA0765-0757, ΔPA2070 and ΔPA5033. The images of biofilms at 200 X magnification were taken by phase-contrast microscopy after 24 h of growth at 37°C in M63-arginine medium. B. Quantification of biofilm formation by measurement of the biofilm-associated crystal violet (OD550). The data shown are the mean ± standard deviation from two separate experiments (each with quadruplicate samples) using the microtitre plate biofilm assay and display no significant difference (p>0.05) among the four groups as determined by one-way ANOVA.
Table 3.
Minimal bactericidal concentrations of antibiotics for PA14 deletion mutants tested with planktonic cells (MBC-P) and biofilm cells (MBC-B) (µg/ml)a.
Table 4.
Antibiotic susceptibility of PA14 deletion mutants assayed in LB broth.
Figure 2.
qPCR analysis of the expression of PA0756, PA2070 and PA5033 in planktonic and biofilm cells.
For each condition, three biological replicate samples were tested in triplicate qPCRs.
Table 5.
Antibiotic susceptibility of PA14 deletion mutants containing pJB866-based plasmids and assayed in LB.
Figure 3.
ΔPA0756-0757, ΔPA2070 and ΔPA5033 are attenuated in a C. elegans slow-killing model.
Slow-killing conditions were used for each strain and death of C. elegans was measured every 24 h for a total of 72 h. Exposure to bacterial lawn represents when L4 or young adult hermaphrodite C. elegans were added to pathogenic plates. Values represent the results from at least three biological replicates. Error bars represent standard deviation, and lack of error bars means a standard deviation of zero.