Figure 1.
Macroscopic observations of the hair cycle.
The hair shafts of murine skin were shaven by animal clippers beforehand. A: The significant area of color in the mouse dorsal skin was surrounded by a red line. B: The corresponding skin color gray-scale ratio on day 9 was shown on the left and day 19 on the right. Data were presented as mean ± SEM, n = 9 in each group, * P<0.05 compared with control group, † P<0.05 compare with CRS group.
Figure 2.
Effects of stress on the hair cycle stage.
A: A representative area of each group on day 9 and day 19 after depilation with the majority of hair follicles. Original magnification was×100. B: The results of hair follicles score for day 9 were shown on the left and the data for day 19 on the right. The Y axis depicted histometric score assessed anagen induction. For each mouse a minimum of 10 individual visual fields were assigned to define hair cycle stages. Data were presented as mean ± SEM, n = 9 in each group, * P<0.05 compared with control group, † P<0.05 compared with CRS group.
Figure 3.
Effects of CRS on mice body weight gain and plasma corticosterone concentrations.
A: On day 4, 9, 14, and 19 after depilation, body weight gain was significantly inhibited in CRS group compared with control group. B: The plasma corticosterone concentrations of mice for day 9 were shown on the left and the data for day 19 on the right. Data are presented as mean ± SEM, n = 9 in each group, * P<0.05 compared with control group.
Figure 4.
CRS induces skin tissue oxidative stress.
A: TBARS levels in mice skin tissue on day 9 shown on the left and on day 19 shown on the right. B: SOD activities in skin on day 9 shown on the left and on day 19 shown on the right. C: GSH-Px activities in skin on day 9 shown on the left and on day 19 shown on the right. Data were presented as mean ± SEM, n = 9 in each group, * P<0.05 compared with control group, † P<0.05 compared with CRS group.
Figure 5.
CRS increases amount of SP+ nerve fibers in dermis on day 9.
A representative area of dermis in each group on day 9 after depilation was shown. The nerve fiber was stained continuous green linear and SP+ nerve fiber was stained red. Original magnification was×500. n = 6 in each group,
Figure 6.
CRS increases amount of SP+ nerve fibers in dermis on day 19.
A representative area of dermis in each group on day 19 after depilation was shown. The nerve fiber was stained continuous green linear and SP+ nerve fiber was stained red. Original magnification was×500. n = 6 in each group.
Figure 7.
Effects of CRS on skin mast cell activation in dermis.
A: A representative area of dermis in each group on day 9 and day 19 after depilation was shown. Original magnificaiton was×400. Dark blue stained cells are mast cells. B: The degranulated mast cells in each group on day 9 were shown on the left and day 19 on the right. Data were presented as mean ± SEM, n = 6 in each group, * P<0.05 compared with control group, † P<0.05 compared with CRS group.
Figure 8.
Schematic diagram of oxidative stress affects hair follicles cycling induced by psychological stress.
Oxidative stress delay the anagen of hair cycle and activate SP-Mast cell pathway, and SP also affects oxidative stress in skin.