Table 1.
Comparison of 454 transcriptome assembly against reference protein databases.
Figure 1.
Scanning electron microscopy of ragweed pollen exposed to different ozone concentrations: (a–c) 40 ppb ozone, (d–f) 80 ppb ozone.
Bars: a, d 50 µm; b, e 20 µm; c, f 5 µm.
Figure 2.
ATR-FTIR spectra of ozone- and control-treated Ambrosia artemisiifolia pollen.
A shows the averaged absorption spectra of ozone-treated (black; n = 5) and control pollen (grey; n = 6) in a range of wavenumbers between 900–3050 cm−1. Standard errors for these spectra are stated at the bottom of part A in black and grey, respectively. B indicates the Δ-absorption of ozone spectra minus control spectra. Small letters stated in part B are further described in Table 2.
Table 2.
ATR-FTIR analysis – explanation of labels from Figure 2.
Figure 3.
Accumulation of total phenolics analysed.
Plants were fumigated with 40 ppb (control) or 80 ppb ozone; bars indicate ± SD; n = 7.
Figure 4.
Common and differently matched Arabidopsis genes. Number of Arabidopsis genes that matched by either sequences of both groups or sequences of one group exclusively.
Figure 5.
Interesting BIN terms detected by MapMan [46].
Arabidopsis sequence matches were grouped due to their log2-fold-change value to three groups: black = induction by ozone (log2>1), grey = common (log2≤ −1; log2≥1), white = repression by ozone (log2< −1). For these groups, the number of transcripts according to the BIN term is stated. Only matches with e-value ≤1 e−5 and RPKM ≥7 were taken into account.
Figure 6.
Log2-fold changes (RPKMozone/RPKMcontrol) of known Ambrosia artemisiifolia allergens.
All data presented are isotig specific and normalized on RPKM.
Table 3.
Allergens detected by 454-sequencing in Ambrosia artemisiifolia pollen.
Figure 7.
Box-Plot of a direct ELISA for the major allergen Amb a 1 of Ambrosia pollen extracts.
50 µg ml−1 total protein were coated. Cross indicates the mean value; ozone n = 14, control n = 19.